AT-1

The AT-1 cell line is a subclone of the parental R3327 rat prostate adenocarcinoma cell line. This particular cell line was derived from the Dunning model, which is a well-established model used to study prostate cancer. The AT-1 subclone is characterized by its relatively slow growth rate and low metastatic potential compared to other subclones derived from the same tumor, such as the MatLyLu (high metastatic potential) and AT-2 (moderate metastatic potential) cell lines. This makes the AT-1 cell line particularly useful for studies focused on the biology of non-metastatic or minimally invasive tumors.

In research settings, the AT-1 cell line has been utilized extensively to investigate the mechanisms of prostate cancer progression and to assess the efficacy of therapeutic agents. The cells generally exhibit a cuboidal morphology and are adherent. They have been shown to respond to hormonal manipulations, which mimics the hormonal responses seen in clinical prostate cancer. Studies using the AT-1 cell line have contributed to a better understanding of the interactions between tumor cells and the microenvironment, angiogenesis, and the molecular pathways involved in cancer progression. Importantly, the AT-1 cell line has been a valuable tool in the development of therapeutic strategies that are less focused on metastasis and more on primary tumor growth and local invasion.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.