CAL-62

The CAL-62 cell line was established from the right lobe of the thyroid gland of a 70-year-old Caucasian woman in 1988 and has been extensively used in the study of thyroid anaplastic carcinoma. These human epithelial-like cells exhibit a distinctive monolayer growth pattern and demonstrate pronounced tumorigenic properties, making them a significant model for in vivo studies of thyroid cancer progression. When transplanted into immunodeficient nude mice, CAL-62 cells have shown a robust capability to form tumors, providing a practical and effective model to analyze tumor dynamics and evaluate potential therapeutic strategies in real-time biological settings.

Characterized by a rapid proliferation rate with a doubling time of approximately 24 hours, CAL-62 enables accelerated research outputs in studies that are time-sensitive, enhancing the efficiency of experimental workflows in cancer research. Genetic characterization of this cell line reveals the presence of the KRAS p.G12R mutation and alterations at the 9p21.3 locus, indicating complex genetic underpinnings associated with thyroid anaplastic carcinoma. This cell line’s stable epithelial phenotype and inherent radioresistance further underscore its utility in uncovering novel insights into the pathophysiology of aggressive thyroid cancers and in the development of new therapeutic modalities. The unique attributes of CAL-62, including its aggressive tumor-forming ability and genetic markers, make it a pivotal resource in the ongoing efforts to better understand and treat thyroid anaplastic carcinoma.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.