SNU-1

The SNU-1 cell line is derived from the gastric carcinoma of a human adult and is widely utilized in gastric cancer research. This cell line provides an important model for studying the molecular and cellular mechanisms underlying gastric adenocarcinoma, a common and often deadly form of stomach cancer. SNU-1 cells are particularly valuable for investigating the genetic alterations and signaling pathways involved in the pathogenesis of gastric cancer, as well as for developing and testing new therapeutic strategies.

SNU-1 cells exhibit an epithelial morphology and are characterized by the expression of markers typical of gastric epithelial cells and adenocarcinoma, such as carcinoembryonic antigen (CEA) and cytokeratins. They are often used in studies exploring the role of oncogenes, tumor suppressor genes, and other molecular factors in gastric cancer progression. Researchers employ SNU-1 cells to assess the efficacy and mechanisms of action of chemotherapeutic agents, targeted therapies, and combination treatments. Additionally, SNU-1 cells serve as a model for understanding the tumor microenvironment and the interactions between cancer cells and stromal cells. The relevance of the SNU-1 cell line in gastric cancer research highlights its importance in advancing our knowledge of this malignancy and in the development of effective treatments for gastric cancer patients.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.