- Phenol-red free
- No
- Serum content
- Serum-free
- Recommended
- 6,000 cells/cm² (initial seeding up to approx. 90 % confluence)
Documents
Mammary Epithelial Cell Growth Medium
Product · Product number 850210
Serum-free medium for human mammary epithelial cells
01Description
Mammary Epithelial Cell Growth Medium is a serum-free medium for the culture of human mammary epithelial cells. We offer the medium as 500 ml basal medium, on its own or together with the supplements, either as individual components or as a premixed Supplement-Mix. The individual components allow single factors to be adjusted or left out, while the Supplement-Mix is added in a single step. Supplements are required for use, and the individual supplement components are also available separately.
02Product details
- Phenol-red free
- No
- Serum content
- Serum-free
- Recommended
- 6,000 cells/cm² (initial seeding up to approx. 90 % confluence)
- Storage and stability
- Store the basal medium at 2–8 °C, protected from light, and the supplements at −20 °C. Avoid repeated freeze-thaw cycles of the supplements. Once supplemented, the medium can be kept at 2–8 °C, protected from light, for up to one month. Do not freeze the medium.
- Specification
- pH 7.6, osmolality 285 ± 10 mOsm/kg
- Supplement components
- L-glutamine: 4 mM Bovine pituitary extract (BPE): 50 µg/ml Human recombinant EGF: 10 ng/ml Hydrocortisone: 0.5 µg/ml Human recombinant insulin: 5 µg/ml Antibiotics (optional): gentamicin 50 µg/ml, amphotericin B 50 ng/ml
- Composition table
Supplement volumes for 500 ml of basal medium. The same components are also available premixed, together with the basal medium (Cat. No. 850184).
Component
Volume
Cat. No.
L-glutamine
10 ml
850121
Bovine pituitary extract (BPE)
2 ml
850123
Human recombinant EGF
1 ml
850115
Hydrocortisone
0.5 ml
850129
Human recombinant insulin
0.5 ml
850140
Antibiotics (optional)
3.5 ml
850119
Total (without antibiotics)
14 ml