LCLC-97TM1

The LCLC-97TM1 cell line is derived from a large cell lung carcinoma (LCLC) and was established using a xenograft approach, specifically from the first nude mouse passage of a primary large cell carcinoma. This cell line exhibits densely packed epithelioid islets in culture, with cell borders that are typically indistinguishable under standard microscopic examination. Unlike many other cell lines, LCLC-97TM1 cultures do not generally reach confluency, which may be attributed to their unique growth patterns.

Cytologically, LCLC-97TM1 cells are characterized by a large, single, round nucleus that contains one or two prominent nucleoli, and an evenly distributed chromatin pattern. This nuclear morphology is indicative of the aggressive nature often associated with large cell lung carcinoma. The cell line is also noted for being PAS (Periodic Acid-Schiff) negative and showing no reactivity with Alcian blue staining, which are consistent with the characteristics observed in both the original tumor and the xenograft derived from the cell line.

Chromosomal analysis of LCLC-97TM1 reveals its complex karyotype, which is typical of large cell carcinomas and suggests significant genetic instability. This genetic profile, combined with its distinct morphological features, makes LCLC-97TM1 a valuable model for studying the pathobiology of large cell lung carcinoma, particularly in the context of tumorigenesis, metastasis, and therapeutic response in non-small cell lung cancer (NSCLC).

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.