C3H/10T1/2, Clone 8

C3H/10T1/2，克隆 8 细胞系是从 C3H 小鼠胚胎组织中提取的小鼠成纤维细胞系。这种细胞系在生物研究中被广泛使用，因为它能在适当药剂的作用下分化成多种细胞类型。C3H/10T1/2 细胞具有成纤维细胞的典型特征，但在特定的实验条件下也有转化为脂肪细胞、软骨细胞或成骨细胞的显著能力。这使它们成为研究间充质分化、组织工程和癌变的宝贵模型。

这些细胞在研究致癌物质的作用机制和细胞转化的遗传调控方面尤其引人注目。C3H/10T1/2，克隆 8 细胞对接触抑制敏感，并能在标准培养条件下保持稳定的表型，这对实验结果的可重复性至关重要。此外，C3H/10T1/2 对各种化学和环境刺激的反应能力使其成为毒理学研究的绝佳模型，可用于研究各种物质对细胞行为和分化途径的影响。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

有关详细信息，请参阅我们的 <a href="" /Who-we-are/Warranty/"" hreflang=""x-default"">保修声明 。