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HBZY-1
Tissue
肾脏
Synonyms
HBZY 1, HBZY1

Documents

HBZY-1

Cell line · Product number 305206

HBZY-1 cells are primary cells isolated from the glomerulus of rat kidneys, specifically from mesangial cells. These cells are highly regarded in scientif...

US$395.00excl. tax and shipping
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Lot
Recommended (freshest lot)
  • CoA
1

01General information

Description

HBZY-1 细胞是从大鼠肾小球,特别是从系膜细胞中分离出来的原始细胞。由于其来源和功能,这些细胞在科学研究中备受推崇。肾小球是肾脏的重要结构,对血液过滤和净化至关重要。间质细胞在维持这一特殊肾单位的结构和功能方面发挥着重要作用。因此,HBZY-1 细胞为研究肾脏生物学的复杂性和增进我们对肾脏相关疾病的了解提供了一个宝贵的模型。

在各种科学研究中,HBZY-1 细胞使研究人员能够深入研究肾间质细胞的功能和肾脏疾病的发病机制。因此,HBZY-1 细胞是研究肾脏生物学中至关重要的细胞过程、信号通路和分子相互作用的重要工具。利用这些体外细胞可以深入了解支配间质细胞行为的分子机制,增强我们对它们在肾脏功能和疾病中作用的认识。

此外,HBZY-1 细胞还可用于肾脏疾病(如肾小球肾炎和糖尿病肾病)的病理生理学研究。这些细胞可在模拟疾病状态的实验条件下进行实验,为研究导致肾脏病理的分子事件提供了一个平台。这种能力使 HBZY-1 细胞在药物发现和旨在治疗肾脏相关疾病的治疗干预措施的开发中发挥了重要作用,有可能在患者护理和治疗策略方面取得重大进展。

Tissue
肾脏
Synonyms
  • HBZY 1
  • HBZY1

02Classification

Organism
Rat
Growth properties
Adherent
Biosafety level
1

03Characteristics

Morphology
上皮细胞

04Identifiers

Citation
HBZY-1(Cytion 产品编号 305206)
NCBI TaxID
10116
Cellosaurus
CVCL_7213

05Handling and culture

Culture medium
DMEM,w:4.5 克/升葡萄糖,w:4 毫摩尔 L-谷氨酰胺,w:3.7 克/升 NaHCO3,w:1.0 毫摩尔丙酮酸钠(Cytion 编号 820300a)。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% FBS
Dissociation reagent
Accutase
Subculturing
去除粘附细胞上的旧培养基,用不含钙和镁的 PBS 冲洗。T25 烧瓶用 3-5 毫升 PBS,T75 烧瓶用 5-10 毫升。然后用 Accutase 完全覆盖细胞,T25 烧瓶用 1-2 毫升,T75 烧瓶用 2.5 毫升。让细胞在室温下孵育 8-10 分钟,使其分离。孵育后,用 10 毫升培养基轻轻混合细胞使其重悬,然后用 300xg 离心 3 分钟。弃去上清液,用新鲜培养基重悬细胞,并将其转移到已装有新鲜培养基的新烧瓶中。
Fluid renewal
每周 2 至 3 次
Thawing and culturing
  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

  7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

  8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

06Quality control and cryopreservation

Sterility

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染,每天都要对细胞培养物进行目视检查。

Freeze medium
我们使用完全生长培养基(包括 FBS)+10% DMSO 或 CM-1(Cytion 商品目录编号 800100)作为冷冻保存培养基,以获得足够的解冻后存活率,CM-1(Cytion 商品目录编号 800100)含有优化的渗透保护剂和代谢稳定剂,可提高恢复能力并减少冷冻引起的应激。
Storage conditions

如需长期保存,可将样品瓶放入气相液氮中,温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运,并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后,应立即检查容器,并立即将小瓶转移到适当的储藏室。