HCC827

HCC827 是一种人类非小细胞肺癌细胞系，来源于一名中年女性患者的肺腺癌。这些细胞呈现上皮形态，常用于表皮生长因子受体（EGFR）的相关研究。HCC827 细胞对酪氨酸激酶抑制剂（TKIs）特别敏感，尤其是那些针对表皮生长因子受体突变的抑制剂。这一特性使其成为研究肺癌对表皮生长因子受体抑制剂敏感性的分子机制以及测试靶向表皮生长因子受体依赖途径的新治疗药物疗效的宝贵模型。

该细胞系还可用于探索靶向疗法获得性耐药的机制，这是治疗肺癌的一大挑战。利用 HCC827 细胞进行的研究有助于更好地了解使表皮生长因子受体抑制剂产生耐药性的基因和表观遗传学改变。这些发现对开发克服肺癌患者耐药性和改善治疗效果的策略具有重要意义。此外，HCC827 细胞系还是研究肺腺癌更广泛的细胞和分子状况的工具，包括研究细胞信号传导、肿瘤微环境和癌症转移。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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