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Hs 611.T
Tissue
淋巴结
Disease
霍奇金淋巴瘤
Metastatic site
淋巴结
Synonyms
Hs-611-T, Hs611T, HS611T

Documents

Hs 611.T

Cell line · Product number 305915

Hs 611.T is a human classical Hodgkin lymphoma (cHL) cell line established from the lymph node of a 48-year-old Caucasian female. The line is derived from...

US$700.00excl. tax and shipping
In stock
Lot
Recommended (freshest lot)
  • CoA
1

01General information

Description

Hs 611.T 是一株源自一名 48 岁白人女性淋巴结的人类经典型霍奇金淋巴瘤(cHL)细胞系。该细胞系源自霍奇金淋巴瘤中 T 细胞丰富的结节硬化亚型,这是最常见的 cHL 亚型之一。 Hs 611.T 以悬浮方式增殖,呈现淋巴母细胞样形态。由于肿瘤组织中恶性霍奇金-里德-斯特恩伯格(HRS)细胞稀少,源自霍奇金淋巴瘤的细胞系相对罕见;Hs 611.T 为研究经典型霍奇金淋巴瘤的生物学特性提供了一个宝贵的体外模型。

Hs 611.T 可应用于霍奇金淋巴瘤研究,包括 HRS 细胞信号传导、NF-κB 通路活性、JAK-STAT 通路调控、表面抗原表达(包括 CD30、CD15、 CD25)的研究,以及对霍奇金淋巴瘤靶向治疗(如布伦图西马布维多汀(抗CD30 ADC))的反应研究。 该细胞系还用于免疫学检测,以评估T细胞抑制、细胞因子分泌以及经典霍奇金淋巴瘤特有的肿瘤微环境相互作用。作为BSL-2级细胞系,其操作需采取适当的防护措施。

Tissue
淋巴结
Disease
霍奇金淋巴瘤
Metastatic site
淋巴结
Applications
霍奇金淋巴瘤研究;NF-κB和JAK-STAT通路研究;CD30/CD15/CD25表达检测;布伦图西马布维多汀疗效研究;肿瘤微环境;免疫疗法研究
Synonyms
  • Hs-611-T
  • Hs611T
  • HS611T

02Classification

Organism
Human
Sex
Female
Growth properties
Suspension
Biosafety level
1

03Characteristics

Morphology
淋巴母细胞样
Cell type
B 淋巴细胞
Doubling time
约24至48小时

04Identifiers

Citation
Hs 611.T(Cytion 产品编号 305915)
NCBI TaxID
9606
Cellosaurus
CVCL_0823

05Handling and culture

Culture medium
DMEM,w:4.5 克/升葡萄糖,w:4 毫摩尔 L-谷氨酰胺,w:3.7 克/升 NaHCO3,w:1.0 毫摩尔丙酮酸钠(Cytion 编号 820300a)。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% FBS
Dissociation reagent
无
Subculturing
用新鲜的预热完全培养基将培养物稀释至3–5 × 10⁵ 个细胞/毫升。需要更换培养基时,可进行离心处理(300×g,5 分钟)。传代前应计数存活细胞,以保持最佳密度。无需进行酶解分离。
Split ratio
1 至 3
Seeding density
2 至 4 × 10⁵ 个细胞/毫升
Fluid renewal
每 2 至 3 天
Thawing and culturing
  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

  7. Follow the procedure described under Post-Thaw Recovery

06Quality control and cryopreservation

Freeze medium
作为冷冻保存培养基,我们使用完全生长培养基+10% DMSO,以获得足够的解冻后存活率。
Storage conditions

如需长期保存,可将样品瓶放入气相液氮中,温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运,并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后,应立即检查容器,并立即将小瓶转移到适当的储藏室。

07Donor

Donor age
48 年
Ethnicity
高加索人