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MA-Balb
Tissue
乳房
Disease
小鼠乳腺恶性肿瘤
Synonyms
Ma-Balb

Documents

MA-Balb

Cell line · Product number 400270

Ma-Balb is a mouse cell line established in vitro from a solid, spontaneously occurring mammary carcinoma in a female BALB/c mouse. This cell line origina...

US$800.00excl. tax and shipping
In stock
Lot
Recommended (freshest lot)
  • CoA
1

01General information

Description

Ma-Balb 是一种小鼠细胞系,由雌性 BALB/c 小鼠自发形成的实体乳腺癌体外培养而成。该细胞系来源于一只年轻 BALB/c 小鼠乳腺区域的豆粒大小的实体瘤。Ma-Balb细胞在癌症研究中具有重要意义,尤其是在研究乳腺肿瘤方面,因为它们来源于一种已知易患此类癌症的肿瘤株。

Ma-Balb 细胞系具有类似成纤维细胞的形态,为研究乳腺癌的细胞和分子机制提供了一个强大的模型。研究人员利用这些细胞探索导致肿瘤发生的遗传和环境因素,从而加深对癌症生物学的理解。此外,Ma-Balb 细胞还有助于测试新的抗癌疗法,深入了解药物的疗效和毒性。它们还与免疫学研究有关,有助于阐明癌细胞与免疫系统之间的相互作用,从而促进免疫疗法的发展。

Tissue
乳房
Disease
小鼠乳腺恶性肿瘤
Synonyms
  • Ma-Balb

02Classification

Organism
Mouse
Sex
Female
Growth properties
Adherent
Biosafety level
1

03Characteristics

Morphology
上皮样
Cell type
成纤维细胞
Tumourigenic
是,在 Balb/c 小鼠中

04Identifiers

Citation
MA-Balb(Cytion 目录号 400270)
NCBI TaxID
10090
Cellosaurus
CVCL_5795

05Expression and susceptibility

Viruses
MAP 测试呈阴性。

06Handling and culture

Culture medium
DMEM,w:4.5 克/升葡萄糖,w:4 毫摩尔 L-谷氨酰胺,w:3.7 克/升 NaHCO3,w:1.0 毫摩尔丙酮酸钠(Cytion 编号 820300a)。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% FBS
Dissociation reagent
Accutase
Subculturing
去除粘附细胞上的旧培养基,用不含钙和镁的 PBS 冲洗。T25 烧瓶用 3-5 毫升 PBS,T75 烧瓶用 5-10 毫升。然后用 Accutase 完全覆盖细胞,T25 烧瓶用 1-2 毫升,T75 烧瓶用 2.5 毫升。让细胞在室温下孵育 8-10 分钟,使其分离。孵育后,用 10 毫升培养基轻轻混合细胞使其重悬,然后用 300xg 离心 3 分钟。弃去上清液,用新鲜培养基重悬细胞,并将其转移到已装有新鲜培养基的新烧瓶中。
Fluid renewal
每周 2 至 3 次
Post-thaw recovery
24 至 48 小时
Thawing and culturing
  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

  7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

  8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

07Quality control and cryopreservation

Sterility

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染,每天都要对细胞培养物进行目视检查。

Freeze medium
我们使用完全生长培养基(包括 FBS)+10% DMSO 或 CM-1(Cytion 商品目录编号 800100)作为冷冻保存培养基,以获得足够的解冻后存活率,CM-1(Cytion 商品目录编号 800100)含有优化的渗透保护剂和代谢稳定剂,可提高恢复能力并减少冷冻引起的应激。
Storage conditions

如需长期保存,可将样品瓶放入气相液氮中,温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运,并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后,应立即检查容器,并立即将小瓶转移到适当的储藏室。

08Donor

Breed / subspecies
BALB/c
Donor age
1 年