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SW527
Tissue
乳房;乳腺
Disease
乳腺腺癌
Synonyms
SW-527, SW 527

Documents

SW527

Cell line · Product number 300640

The SW527 cell line is a human breast carcinoma cell line derived from a Caucasian adult patient. It was established in the early 1970s and has been inclu...

US$550.00excl. tax and shipping
In stock
Lot
Recommended (freshest lot)
  • CoA
1

01General information

Description

SW527细胞系是一种源自白种成年患者的人类乳腺癌细胞系。该细胞系建立于20世纪70年代初期,曾被用于免疫缺陷模型中肿瘤发生特性的基础研究。 在某项研究中,SW527细胞经皮下接种6×10⁶个细胞后成功在裸鼠体内形成肿瘤,证实其恶性起源。对形成的肿瘤进行组织病理学分析显示,其特征与原始人类癌瘤一致,确认了其作为乳腺癌模型的适用性。

SW527经鉴定为G6PD B型肿瘤来源细胞系,该分类有助于排除HeLa细胞污染风险——这在历史细胞系收藏中是关键关注点。尽管如此,近期大规模数据集中对SW527的全面分子或免疫学特征分析仍显不足。

总体而言,SW527仍属经验证的乳腺癌模型,其有效性主要基于体内致瘤性数据。若能补充分子特征分析,将有助于拓展该细胞系在机制研究及药物发现领域的应用价值。

Tissue
乳房;乳腺
Disease
乳腺腺癌
Synonyms
  • SW-527
  • SW 527

02Classification

Organism
Human
Sex
Female
Growth properties
Adherent
Biosafety level
1

03Characteristics

Morphology
上皮细胞
Cell type
上皮细胞

04Identifiers

Citation
SW527(Cytion 目录号 300640)
NCBI TaxID
9606
Cellosaurus
CVCL_3799

05Expression and susceptibility

Mutational profile
突变:p.Gln1338Ter,同型;突变:p.Gly12Val,同型;突变:p.Arg273His,异型;突变:p.Pro309Ser,异型

06Handling and culture

Culture medium
DMEM:Ham's F12(1:1),w: 3.1 g/L 葡萄糖,w: 2.5 mM L-谷氨酰胺,w: 15 mM HEPES,w: 0.5 mM 丙酮酸钠,w: 1.2 g/L NaHCO3(Cytion 文章编号 820400a)。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% FBS
Dissociation reagent
Accutase
Thawing and culturing
  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

  7. Follow the procedure described under Post-Thaw Recovery

07Quality control and cryopreservation

Freeze medium
作为冷冻保存培养基,我们使用完全生长培养基+10% DMSO,以获得足够的解冻后存活率。
Storage conditions

如需长期保存,可将样品瓶放入气相液氮中,温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运,并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后,应立即检查容器,并立即将小瓶转移到适当的储藏室。

08Donor

Donor age
70岁
Ethnicity
高加索人