SW527
Cell line · Product number 300640
The SW527 cell line is a human breast carcinoma cell line derived from a Caucasian adult patient. It was established in the early 1970s and has been inclu...
Recommended (freshest lot)
01General information
- Description
SW527细胞系是一种源自白种成年患者的人类乳腺癌细胞系。该细胞系建立于20世纪70年代初期,曾被用于免疫缺陷模型中肿瘤发生特性的基础研究。 在某项研究中,SW527细胞经皮下接种6×10⁶个细胞后成功在裸鼠体内形成肿瘤,证实其恶性起源。对形成的肿瘤进行组织病理学分析显示,其特征与原始人类癌瘤一致,确认了其作为乳腺癌模型的适用性。
SW527经鉴定为G6PD B型肿瘤来源细胞系,该分类有助于排除HeLa细胞污染风险——这在历史细胞系收藏中是关键关注点。尽管如此,近期大规模数据集中对SW527的全面分子或免疫学特征分析仍显不足。
总体而言,SW527仍属经验证的乳腺癌模型,其有效性主要基于体内致瘤性数据。若能补充分子特征分析,将有助于拓展该细胞系在机制研究及药物发现领域的应用价值。
- Tissue
- 乳房;乳腺
- Disease
- 乳腺腺癌
- Synonyms
- SW-527
- SW 527
02Classification
- Organism
- Human
- Sex
- Female
- Growth properties
- Adherent
- Biosafety level
- 1
03Characteristics
- Morphology
- 上皮细胞
- Cell type
- 上皮细胞
04Identifiers
- Citation
- SW527(Cytion 目录号 300640)
- NCBI TaxID
- 9606
- Cellosaurus
- CVCL_3799
05Expression and susceptibility
- Mutational profile
- 突变:p.Gln1338Ter,同型;突变:p.Gly12Val,同型;突变:p.Arg273His,异型;突变:p.Pro309Ser,异型
06Handling and culture
- Culture medium
- DMEM:Ham's F12(1:1),w: 3.1 g/L 葡萄糖,w: 2.5 mM L-谷氨酰胺,w: 15 mM HEPES,w: 0.5 mM 丙酮酸钠,w: 1.2 g/L NaHCO3(Cytion 文章编号 820400a)。
- Incubation atmosphere
37°C, 5%CO2, 加湿环境。
- Supplements
- 在培养基中添加 10% FBS
- Dissociation reagent
- Accutase
- Thawing and culturing
Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.
Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.
For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.
Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.
Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.
Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.
Follow the procedure described under Post-Thaw Recovery
07Quality control and cryopreservation
- Freeze medium
- 作为冷冻保存培养基,我们使用完全生长培养基+10% DMSO,以获得足够的解冻后存活率。
- Storage conditions
如需长期保存,可将样品瓶放入气相液氮中,温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。
- Shipping conditions
冷冻保存的细胞系用干冰装运,并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后,应立即检查容器,并立即将小瓶转移到适当的储藏室。
08Donor
- Donor age
- 70岁
- Ethnicity
- 高加索人
09Legal and safety
- Safety precautions
- 计划将低温样品储存在液氮中以便将来解冻时,必须遵守严格的安全措施。在将冷冻样本移入或移出液氮罐时,必须穿戴适当的防护手套和防护服,并使用面罩或安全护目镜。这样做是为了降低在移出时因冷冻样品爆炸而造成伤害的风险,因为爆炸可能导致锋利的碎片飞溅。
- Warranty
在Cytion,我们保证产品的质量和活力。 我们保证,从我们的工厂发货后 60 天内,我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。
有关详细信息,请参阅我们的 <a href="" /Who-we-are/Warranty/"" hreflang=""x-default"">保修声明 。
- Material Transfer Agreement required
- Yes
