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SW756
Tissue
子宫、宫颈
Disease
鳞状细胞癌
Synonyms
SW-756, SW 756

Documents

SW756

Cell line · Product number 305588

SW756 is a human cervical carcinoma cell line derived from a primary squamous cell carcinoma of the cervix in an adult female. It was included in early co...

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Lot
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  • CoA
1

01General information

Description

SW756 是一种人类宫颈癌细胞系,来源于一名成年女性的宫颈原发性鳞状细胞癌。它被纳入早期的综合细胞系特征鉴定工作中,并根据葡萄糖-6-磷酸脱氢酶(G6PD)同工酶分型被确认为不含 HeLa 污染,该分型证实它是 B 型细胞,而不像 HeLa 细胞是 A 型细胞。

SW756 被归类为鳞状细胞癌,已被用于各种基因组和蛋白质组分析研究,包括大规模药物基因组学和功能基因组学数据集。在 ProCan-DepMapSanger 数据集等蛋白质组分析工作中,SW756 有助于更广泛地了解蛋白质表达调控,特别是转录后修饰和药物反应相关性。这些数据集表明,与其他细胞系一样,SW756 的蛋白质组数据也能揭示在转录组水平上并不总是很明显的系特异性表达模式和调控机制,突出了它在多组学综合分析中的作用。

Tissue
子宫、宫颈
Disease
鳞状细胞癌
Synonyms
  • SW-756
  • SW 756

02Classification

Organism
Human
Sex
Female
Growth properties
Adherent
Biosafety level
1

03Characteristics

Doubling time
1.6 天

04Identifiers

Citation
SW756(Cytion 目录号 305588)
NCBI TaxID
9606
Cellosaurus
CVCL_1727

05Expression and susceptibility

Mutational profile
突变:p.Gly12Cys,杂合子

06Handling and culture

Culture medium
DMEM,w:4.5 克/升葡萄糖,w:4 毫摩尔 L-谷氨酰胺,w:3.7 克/升 NaHCO3,w:1.0 毫摩尔丙酮酸钠(Cytion 编号 820300a)。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% FBS
Dissociation reagent
Accutase
Thawing and culturing
  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

  7. Follow the procedure described under Post-Thaw Recovery

07Quality control and cryopreservation

Freeze medium
作为冷冻保存培养基,我们使用完全生长培养基+10% DMSO,以获得足够的解冻后存活率。
Storage conditions

如需长期保存,可将样品瓶放入气相液氮中,温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运,并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后,应立即检查容器,并立即将小瓶转移到适当的储藏室。

08Donor

Donor age
46 年
Ethnicity
高加索人