SW756
Cell line · Product number 305588
SW756 is a human cervical carcinoma cell line derived from a primary squamous cell carcinoma of the cervix in an adult female. It was included in early co...
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01General information
- Description
SW756 是一种人类宫颈癌细胞系,来源于一名成年女性的宫颈原发性鳞状细胞癌。它被纳入早期的综合细胞系特征鉴定工作中,并根据葡萄糖-6-磷酸脱氢酶(G6PD)同工酶分型被确认为不含 HeLa 污染,该分型证实它是 B 型细胞,而不像 HeLa 细胞是 A 型细胞。
SW756 被归类为鳞状细胞癌,已被用于各种基因组和蛋白质组分析研究,包括大规模药物基因组学和功能基因组学数据集。在 ProCan-DepMapSanger 数据集等蛋白质组分析工作中,SW756 有助于更广泛地了解蛋白质表达调控,特别是转录后修饰和药物反应相关性。这些数据集表明,与其他细胞系一样,SW756 的蛋白质组数据也能揭示在转录组水平上并不总是很明显的系特异性表达模式和调控机制,突出了它在多组学综合分析中的作用。
- Tissue
- 子宫、宫颈
- Disease
- 鳞状细胞癌
- Synonyms
- SW-756
- SW 756
02Classification
- Organism
- Human
- Sex
- Female
- Growth properties
- Adherent
- Biosafety level
- 1
03Characteristics
- Doubling time
- 1.6 天
04Identifiers
- Citation
- SW756(Cytion 目录号 305588)
- NCBI TaxID
- 9606
- Cellosaurus
- CVCL_1727
05Expression and susceptibility
- Mutational profile
- 突变:p.Gly12Cys,杂合子
06Handling and culture
- Culture medium
- DMEM,w:4.5 克/升葡萄糖,w:4 毫摩尔 L-谷氨酰胺,w:3.7 克/升 NaHCO3,w:1.0 毫摩尔丙酮酸钠(Cytion 编号 820300a)。
- Incubation atmosphere
37°C, 5%CO2, 加湿环境。
- Supplements
- 在培养基中添加 10% FBS
- Dissociation reagent
- Accutase
- Thawing and culturing
Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.
Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.
For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.
Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.
Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.
Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.
Follow the procedure described under Post-Thaw Recovery
07Quality control and cryopreservation
- Freeze medium
- 作为冷冻保存培养基,我们使用完全生长培养基+10% DMSO,以获得足够的解冻后存活率。
- Storage conditions
如需长期保存,可将样品瓶放入气相液氮中,温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。
- Shipping conditions
冷冻保存的细胞系用干冰装运,并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后,应立即检查容器,并立即将小瓶转移到适当的储藏室。
08Donor
- Donor age
- 46 年
- Ethnicity
- 高加索人
09Legal and safety
- Safety precautions
- 计划将低温样品储存在液氮中以便将来解冻时,必须遵守严格的安全措施。在将冷冻样本移入或移出液氮罐时,必须穿戴适当的防护手套和防护服,并使用面罩或安全护目镜。这样做是为了降低在移出时因冷冻样品爆炸而造成伤害的风险,因为爆炸可能导致锋利的碎片飞溅。
- Warranty
在Cytion,我们保证产品的质量和活力。 我们保证,从我们的工厂发货后 60 天内,我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。
有关详细信息,请参阅我们的 <a href="" /Who-we-are/Warranty/"" hreflang=""x-default"">保修声明 。
- Material Transfer Agreement required
- Yes
