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SW756
Tissue
子宮、子宮頸
Disease
鱗狀細胞癌
Synonyms
SW-756, SW 756

Documents

SW756

Cell line · Product number 305588

SW756 is a human cervical carcinoma cell line derived from a primary squamous cell carcinoma of the cervix in an adult female. It was included in early co...

US$550.00excl. tax and shipping
In stock
Lot
Recommended (freshest lot)
  • CoA
1

01General information

Description

SW756 是一種源自成年女性原發性子宮頸鱗狀細胞癌的人類子宮頸癌細胞系。 該細胞系曾被納入早期全面的細胞系特性分析研究,並經葡萄糖-6-磷酸脫氫酶(G6PD)同工酶分型驗證,確認其不含 HeLa 細胞污染——其同工酶型為 B 型,有別於屬 A 型的 HeLa 細胞。這確保了其作為獨立子宮頸癌模型的真實性。

SW756 被歸類為鱗狀細胞癌,並已被應用於各種基因組學和蛋白質組學分析研究,包括大規模的藥物基因組學和功能基因組學數據集。 在蛋白質組學分析工作中(例如 ProCan-DepMapSanger 資料集),SW756 有助於更廣泛地理解蛋白質表達調控機制,特別是在轉錄後修飾與藥物反應相關性方面。 此類資料集顯示,SW756 的蛋白質組學資料與其他細胞系一樣,能夠揭示在轉錄組層面未必顯而易見的譜系特異性表達模式及調控機制,凸顯了其在整合性多組學分析中的實用價值。

Tissue
子宮、子宮頸
Disease
鱗狀細胞癌
Synonyms
  • SW-756
  • SW 756

02Classification

Organism
Human
Sex
Female
Growth properties
Adherent
Biosafety level
1

03Characteristics

Doubling time
1.6 天

04Identifiers

Citation
SW756(Cytion 型號 305588)
NCBI TaxID
9606
Cellosaurus
CVCL_1727

05Expression and susceptibility

Mutational profile
突變:p.Gly12Cys,雜合子

06Handling and culture

Culture medium
DMEM,含 4.5 g/L 葡萄糖、4 mM L-谷氨醯胺、3.7 g/L NaHCO₃ 及 1.0 mM 丙酮酸鈉(Cytion 產品編號 820300a)
Incubation atmosphere

37°C,5% CO₂,加濕環境。

Supplements
在培養基中加入 10% 的胎牛血清(FBS)
Dissociation reagent
Accutase
Thawing and culturing
  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

  7. Follow the procedure described under Post-Thaw Recovery

07Quality control and cryopreservation

Freeze medium
作為冷凍保存培養基,我們使用完全生長培養基加上 10% 的二甲基亞砜(DMSO),以確保解凍後的細胞存活率達到適當水準。
Storage conditions

若需長期保存,請將試管置於溫度約為 −150 至 −196 °C 的氣相液氮中。僅在轉移至液氮之前,可將試管暫時存放於 −80 °C 環境中,作為短期過渡步驟。

Shipping conditions

冷凍保存的細胞系會以乾冰冷藏,並裝於經驗證的隔熱包裝中,包裝內含有足夠的冷媒,以確保在運輸過程中溫度維持在約 −78 °C。收到貨物後,請立即檢查容器,並盡快將試管轉移至適當的儲存場所。

08Donor

Donor age
46年
Ethnicity
高加索人