UM-HMC-3B

UM-HMC-3B 是一种人黏液表皮样癌细胞系，源自一名 73 岁白人女性患者硬腭唾液腺的原发性黏液表皮样癌。 该细胞系属于密歇根大学人类黏液表皮样癌（UM-HMC）细胞库的一部分。 与 UM-HMC-1 一样，UM-HMC-3B 携带 CRTC1-MAML2 基因融合（MECT1-MAML2），这是大多数黏液表皮样癌的致癌驱动基因，由 t(11;19)(q21;p13) 易位所致，并驱动 CREB 和 Notch 通路的激活。

UM-HMC-3B 适用于硬腭和唾液腺黏液表皮样癌生物学研究、CRTC1-MAML2 信号传导研究，以及与其他 UM-HMC 面板细胞系的比较分析。 结合 UM-HMC-1 和该系列的其他成员，UM-HMC-3B 有助于识别不同解剖部位、年龄和性别的黏表皮样癌中共同和不同的分子特征。它还适用于针对融合致癌蛋白及其下游 CREB/Notch 效应因子的药物敏感性检测。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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