Mélanocytes humains, adultes

01Description

02Informations générales

Description

Human Melanocytes, adult are primary pigment-producing melanocytes isolated from adult human skin (epidermis). They are used to study melanogenesis, pigmentation disorders, UV/photobiology, and melanoma biology.

Tissu

Skin (epidermis)

Maladie

Normal

Applications

Melanogenesis and pigmentation research; UV/photobiology; melanoma-related studies

03Classification

Organisme

Humain

Propriétés de croissance

Adepte

Niveau de biosécurité

1

04Caractéristiques

Morphologie

Dendritic / bipolar

Type de cellule

Melanocyte

Temps de doublement

Approximately 48-72 hours

05Identifiants

Référence

Human Melanocytes, adult (Cytion catalog number 300694)

Numéro d'identification fiscale NCBI

9606

06Manipulation et culture

Milieu de culture

Melanocyte Growth Medium

Atmosphère d'incubation

37°C, 5% CO₂, humidified atmosphere.

Compléments alimentaires

Melanocyte growth supplements (e.g., bovine pituitary extract, growth factors); serum as per medium

Réactif de dissociation

Trypsin-EDTA or Accutase

Repiquage

At 70-80% confluence, wash with PBS, detach with Trypsin-EDTA or Accutase, neutralize, centrifuge at 300xg for 3 min, and reseed into fresh medium.

Rapport de fractionnement

1:2 to 1:3

Densité de semis

2.5-5 x 10^3 cells/cm2

Renouvellement des fluides

Every 2-3 days

Décongélation et mise en culture

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

07Contrôle qualité et cryoconservation

Milieu de congélation

As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.

Conditions de stockage

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Conditions de livraison

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

08Aspects juridiques et sécurité

Consignes de sécurité

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

Garantie

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.

Un accord de transfert de matériel est requis

Oui

09Détails du produit

Tableau de composition