인간 멜라닌 세포, 성인

01설명

02일반 정보

설명

Human Melanocytes, adult are primary pigment-producing melanocytes isolated from adult human skin (epidermis). They are used to study melanogenesis, pigmentation disorders, UV/photobiology, and melanoma biology.

티슈

Skin (epidermis)

질병

Normal

응용 분야

Melanogenesis and pigmentation research; UV/photobiology; melanoma-related studies

03분류

생물체

인간

성장 특성

준수자

생물안전 등급

1

04특징

형태학

Dendritic / bipolar

세포 유형

Melanocyte

배가 시간

Approximately 48-72 hours

05식별자

인용

Human Melanocytes, adult (Cytion catalog number 300694)

NCBI 세금 식별번호

9606

06취급 및 배양

배지

Melanocyte Growth Medium

배양 조건

37°C, 5% CO₂, humidified atmosphere.

건강보조식품

Melanocyte growth supplements (e.g., bovine pituitary extract, growth factors); serum as per medium

분리 시약

Trypsin-EDTA or Accutase

재배양

At 70-80% confluence, wash with PBS, detach with Trypsin-EDTA or Accutase, neutralize, centrifuge at 300xg for 3 min, and reseed into fresh medium.

분할 비율

1:2 to 1:3

파종 밀도

2.5-5 x 10^3 cells/cm2

유체 교체

Every 2-3 days

해동 및 배양

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

07품질 관리 및 냉동 보존

배양액 동결

As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.

보관 조건

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

배송 조건

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

08법률 및 안전

안전 수칙

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

보증

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.

물질 이전 계약서(MTA)가 필요합니다.

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