Човешки меланоцити, възрастни

01Описание

02Обща информация

Описание

Human Melanocytes, adult are primary pigment-producing melanocytes isolated from adult human skin (epidermis). They are used to study melanogenesis, pigmentation disorders, UV/photobiology, and melanoma biology.

Тъкан

Skin (epidermis)

Заболяване

Normal

Приложения

Melanogenesis and pigmentation research; UV/photobiology; melanoma-related studies

03Класификация

Организъм

Човек

Свойства, свързани с растежа

Привърженик

Ниво на биологична безопасност

1

04Характеристики

Морфология

Dendritic / bipolar

Тип клетка

Melanocyte

Време за удвояване

Approximately 48-72 hours

05Идентификатори

Източник

Human Melanocytes, adult (Cytion catalog number 300694)

Данъчен идентификационен номер в NCBI

9606

06Обработка и отглеждане

Хранителна среда

Melanocyte Growth Medium

Атмосфера за инкубация

37°C, 5% CO₂, humidified atmosphere.

Добавки

Melanocyte growth supplements (e.g., bovine pituitary extract, growth factors); serum as per medium

Реагент за дисоциация

Trypsin-EDTA or Accutase

Пресаждане

At 70-80% confluence, wash with PBS, detach with Trypsin-EDTA or Accutase, neutralize, centrifuge at 300xg for 3 min, and reseed into fresh medium.

Коефициент на разделяне

1:2 to 1:3

Плътност на засяването

2.5-5 x 10^3 cells/cm2

Обновяване на течността

Every 2-3 days

Размразяване и култивиране

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

07Контрол на качеството и криоконсервация

Среда за замразяване

As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.

Условия за съхранение

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Условия за доставка

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

08Правни въпроси и безопасност

Мерки за безопасност

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

Гаранция

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.

Необходимо е споразумение за прехвърляне на материали

Да

09Подробности за продукта

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