Melanociti umani, adulti

01Descrizione

02Informazioni generali

Descrizione

Human Melanocytes, adult are primary pigment-producing melanocytes isolated from adult human skin (epidermis). They are used to study melanogenesis, pigmentation disorders, UV/photobiology, and melanoma biology.

Tessuto

Skin (epidermis)

Malattia

Normal

Applicazioni

Melanogenesis and pigmentation research; UV/photobiology; melanoma-related studies

03Classificazione

Organismo

Umano

Proprietà di crescita

Aderente

Livello di biosicurezza

1

04Caratteristiche

Morfologia

Dendritic / bipolar

Tipo di cellula

Melanocyte

Tempo di raddoppio

Approximately 48-72 hours

05Identificatori

Riferimento bibliografico

Human Melanocytes, adult (Cytion catalog number 300694)

Codice fiscale NCBI

9606

06Manipolazione e coltura

Terreno di coltura

Melanocyte Growth Medium

Atmosfera di incubazione

37°C, 5% CO₂, humidified atmosphere.

Integratori

Melanocyte growth supplements (e.g., bovine pituitary extract, growth factors); serum as per medium

Reagente di dissociazione

Trypsin-EDTA or Accutase

Subcoltura

At 70-80% confluence, wash with PBS, detach with Trypsin-EDTA or Accutase, neutralize, centrifuge at 300xg for 3 min, and reseed into fresh medium.

Rapporto di frazionamento

1:2 to 1:3

Densità di semina

2.5-5 x 10^3 cells/cm2

Rinnovo dei fluidi

Every 2-3 days

Scongelamento e coltura

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

07Controllo di qualità e crioconservazione

Terreno di coltura congelato

As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.

Condizioni di conservazione

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Condizioni di spedizione

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

08Aspetti legali e di sicurezza

Misure di sicurezza

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.

Garanzia

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.

È richiesto un accordo di trasferimento di materiale

Sì

09Dettagli sul prodotto

Tabella delle composizioni