Gladde spiercellen uit de menselijke longslagader (HPASMC)

01Beschrijving

02Algemene informatie

Beschrijving

Primary Human Pulmonary Artery Smooth Muscle Cells (HPASMC) are isolated from the pulmonary artery. They are a key model for pulmonary arterial hypertension, including hypoxia-driven proliferation and vascular remodeling.

Hypoxia drives the proliferation of HPASMC, and PDGF-BB shifts them toward a synthetic, migratory phenotype, reproducing key features of pulmonary vascular remodeling. Because the balance of BMP and TGF-β signaling is central to pulmonary arterial hypertension, the cells are also used to study BMPR2-related mechanisms.

Weefsel

Lung (pulmonary artery

Ziekte

Normal pulmonary arterial smooth muscle (non-tumorigenic primary cell)

Plaats van uitzaaiingen

Not applicable (normal, non-tumorigenic primary cell)

Toepassingen

Pulmonary arterial hypertension: BMP/TGF-β balance, proliferation and migration; Hypoxia-induced proliferation, PPARγ and microRNA-21; Long non-coding RNA regulation of migration in pulmonary arterial hypertension; Functional testing of BMPR2 promoter variants in reporter assays; PDGF-BB-induced phenotype switching and metabolomics; Endothelin-1 signaling and proteomics of BMPR2-mutant cells

Synoniemen

• HPASMC
• Human pulmonary artery smooth muscle cells
• provitro 1210613

03Donateur

Leeftijd van de donor

Lot-specific (see CoA)

Etnische afkomst

Lot-specific (see CoA)

04Classificatie

Organisme

Mens

Seks

Lot-specifiek

Groei-eigenschappen

Aanhanger

GGO-status

Zonder genetisch gemodificeerde organismen

Details over de GGO-status

No genetic modification; primary human pulmonary artery smooth muscle cells isolated from normal pulmonary arteries. Not transformed or immortalized.

Bioveiligheidsniveau

1

05Kenmerken

Morfologie

Spindle-shaped (smooth muscle)

Celtype

Primary smooth muscle cells

Tumorverwekkend

No

Verdubbelingstijd

Not specified by AddexBio; typical primary VSMC doubling time 36–72 hours

06Behandeling en kweek

Kweekmedium

Smooth Muscle Cell Growth Medium kit (Cytion article number 850075); Smooth Muscle Cell Growth Medium mix (Cytion article number 850074)

Incubatieomgeving

37°C, 5% CO₂, saturated humidity.

Supplementen

Supplement as specified in the AddexBio SMC supplement kit (refer to specification sheet). For cryopreservation: complete culture medium + 5% (v/v) DMSO.

Dissociatiereagens

Subculture reagents as recommended on the certificate of analysis

Heruitplanten

Subculture the cells at about 75 % confluence. Denser cultures undergo irreversible contact inhibition and stop proliferating. Use the subculture reagents recommended on the certificate of analysis.

Splitsingsverhouding

1 to 3

Zaaidichtheid

More than 4,000 cells/cm²

Vloeistofverversing

24 hours after seeding, then every 48 to 72 hours

Herstel na ontdooiing

Post-thaw viability ≥ 70 % viable cells. More than 80 % confluence within the expected recovery period.

Ontdooien en kweken

1. Thaw the cryovial quickly in a 37 °C water bath, swirling gently. Just before the last ice has melted, move the vial to a laminar flow hood and decontaminate its outer surface.

2. Transfer the cell suspension into a 15 ml centrifuge tube, add 10 ml of Smooth Muscle Cell Growth Medium and centrifuge at 250 × g for 5 minutes.

3. Remove the supernatant and resuspend the cell pellet in 10 ml of Smooth Muscle Cell Growth Medium. Check cell number and viability.

4. Divide the suspension between two 25 cm² culture flasks (5 ml each). Using two flasks reduces the risk of losing all cells if a passage fails.

5. Incubate at 37 °C, 5 % CO₂ and saturated humidity, with the screw caps opened by half a turn for gas exchange.

6. Change the medium after 24 hours to remove unattached cells, then every 48 to 72 hours, using pre-warmed medium only.

7. Subculture the cells at about 75 % confluence. Denser cultures undergo irreversible contact inhibition and stop proliferating. Use the subculture reagents recommended on the certificate of analysis.

8. Recommended seeding density: more than 4,000 cells/cm².

07Kwaliteitscontrole en cryopreservatie

Onvruchtbaarheid

Mycoplasma contamination is excluded by PCR.

To ensure there is no bacterial, fungal or yeast contamination, cultures are subjected to visual inspection.

Kweekmedium invriezen

As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.

Opslagvoorwaarden

For long-term preservation, store vials in liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable for up to one week only.

Verzendvoorwaarden

Cryopreserved primary cells are shipped on dry ice. On receipt, check the cryovial for damage and transfer it without delay to appropriate storage.

08Expressie en vatbaarheid

Eiwit-expressie

Smooth muscle α-actin; calponin; smooth muscle myosin heavy chain

Virussen

Free of HIV, Hepatitis B, Hepatitis C (confirmed negative by AddexBio)

09Identificatiegegevens

Bronvermelding

Human pulmonary artery smooth muscle cells (HPASMC) (Cytion catalog number 300706)

NCBI-belastingnummer

9606

Cellosaurus

Not assigned

10Juridische en veiligheidsaspecten

Veiligheidsmaatregelen

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Primary cells are of human origin, and no known test procedure can ensure the complete absence of infectious agents. Handle all products as potentially infectious.

Garantie

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.

Er is een overeenkomst inzake materiaaloverdracht vereist

Ja

11Productgegevens

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