Ludzkie komórki mięśni gładkich tętnicy płucnej (HPASMC)

01Opis

02Informacje ogólne

Opis

Primary Human Pulmonary Artery Smooth Muscle Cells (HPASMC) are isolated from the pulmonary artery. They are a key model for pulmonary arterial hypertension, including hypoxia-driven proliferation and vascular remodeling.

Hypoxia drives the proliferation of HPASMC, and PDGF-BB shifts them toward a synthetic, migratory phenotype, reproducing key features of pulmonary vascular remodeling. Because the balance of BMP and TGF-β signaling is central to pulmonary arterial hypertension, the cells are also used to study BMPR2-related mechanisms.

Chusteczka

Lung (pulmonary artery

Choroba

Normal pulmonary arterial smooth muscle (non-tumorigenic primary cell)

Miejsce przerzutów

Not applicable (normal, non-tumorigenic primary cell)

Zastosowania

Pulmonary arterial hypertension: BMP/TGF-β balance, proliferation and migration; Hypoxia-induced proliferation, PPARγ and microRNA-21; Long non-coding RNA regulation of migration in pulmonary arterial hypertension; Functional testing of BMPR2 promoter variants in reporter assays; PDGF-BB-induced phenotype switching and metabolomics; Endothelin-1 signaling and proteomics of BMPR2-mutant cells

Synonimy

• HPASMC
• Human pulmonary artery smooth muscle cells
• provitro 1210613

03Darczyńca

Wiek dawcy

Lot-specific (see CoA)

Pochodzenie etniczne

Lot-specific (see CoA)

04Klasyfikacja

Organizm

Człowiek

Seks

Specyficzne dla partii

Właściwości związane ze wzrostem

Zwolennik

Status GMO

Bez GMO

Szczegółowe informacje dotyczące statusu GMO

No genetic modification; primary human pulmonary artery smooth muscle cells isolated from normal pulmonary arteries. Not transformed or immortalized.

Poziom bezpieczeństwa biologicznego

1

05Cechy charakterystyczne

Morfologia

Spindle-shaped (smooth muscle)

Typ komórki

Primary smooth muscle cells

Nowotworotwórczy

No

Czas podwojenia

Not specified by AddexBio; typical primary VSMC doubling time 36–72 hours

06Postępowanie i hodowla

Pożywka hodowlana

Smooth Muscle Cell Growth Medium kit (Cytion article number 850075); Smooth Muscle Cell Growth Medium mix (Cytion article number 850074)

Atmosfera inkubacyjna

37°C, 5% CO₂, saturated humidity.

Suplementy

Supplement as specified in the AddexBio SMC supplement kit (refer to specification sheet). For cryopreservation: complete culture medium + 5% (v/v) DMSO.

Odczynnik dysocjacyjny

Subculture reagents as recommended on the certificate of analysis

Hodowla komórek w subkulturze

Subculture the cells at about 75 % confluence. Denser cultures undergo irreversible contact inhibition and stop proliferating. Use the subculture reagents recommended on the certificate of analysis.

Współczynnik podziału

1 to 3

Gęstość wysiewu

More than 4,000 cells/cm²

Odnawianie płynów

24 hours after seeding, then every 48 to 72 hours

Powrót do stanu sprzed rozmrożenia

Post-thaw viability ≥ 70 % viable cells. More than 80 % confluence within the expected recovery period.

Rozmrażanie i hodowla

1. Thaw the cryovial quickly in a 37 °C water bath, swirling gently. Just before the last ice has melted, move the vial to a laminar flow hood and decontaminate its outer surface.

2. Transfer the cell suspension into a 15 ml centrifuge tube, add 10 ml of Smooth Muscle Cell Growth Medium and centrifuge at 250 × g for 5 minutes.

3. Remove the supernatant and resuspend the cell pellet in 10 ml of Smooth Muscle Cell Growth Medium. Check cell number and viability.

4. Divide the suspension between two 25 cm² culture flasks (5 ml each). Using two flasks reduces the risk of losing all cells if a passage fails.

5. Incubate at 37 °C, 5 % CO₂ and saturated humidity, with the screw caps opened by half a turn for gas exchange.

6. Change the medium after 24 hours to remove unattached cells, then every 48 to 72 hours, using pre-warmed medium only.

7. Subculture the cells at about 75 % confluence. Denser cultures undergo irreversible contact inhibition and stop proliferating. Use the subculture reagents recommended on the certificate of analysis.

8. Recommended seeding density: more than 4,000 cells/cm².

07Kontrola jakości i kriokonserwacja

Bezpłodność

Mycoplasma contamination is excluded by PCR.

To ensure there is no bacterial, fungal or yeast contamination, cultures are subjected to visual inspection.

Pożywka do zamrażania

As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.

Warunki przechowywania

For long-term preservation, store vials in liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable for up to one week only.

Warunki wysyłki

Cryopreserved primary cells are shipped on dry ice. On receipt, check the cryovial for damage and transfer it without delay to appropriate storage.

08Ekspresja i podatność

Ekspresja białek

Smooth muscle α-actin; calponin; smooth muscle myosin heavy chain

Wirusy

Free of HIV, Hepatitis B, Hepatitis C (confirmed negative by AddexBio)

09Identyfikatory

Źródło

Human pulmonary artery smooth muscle cells (HPASMC) (Cytion catalog number 300706)

Numer identyfikacji podatkowej NCBI

9606

Cellosaurus

Not assigned

10Kwestie prawne i bezpieczeństwo

Środki bezpieczeństwa

When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Primary cells are of human origin, and no known test procedure can ensure the complete absence of infectious agents. Handle all products as potentially infectious.

Gwarancja

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.

Wymagana umowa o transferze materiałów

Tak

11Szczegóły produktu

Tabela składu