COLO-205

The COLO-205 cell line is a human colorectal adenocarcinoma cell line first established from the metastatic site of the ascites in a 70-year-old Caucasian male. Characterized by its epithelial cell morphology, this cell line is frequently utilized in biomedical research focused on colorectal cancer, particularly in studies related to cancer biology, drug response, and metastatic mechanisms. COLO-205 cells exhibit a hyperdiploid karyotype and are known to form moderately well-differentiated adenocarcinomas when xenografted into immunodeficient mice.

COLO-205 cells express several key oncogenic and tumor suppressor pathways, making them a valuable model for pharmacological testing and cancer research. They are responsive to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) making them suitable for apoptosis studies. Furthermore, these cells have been used extensively to investigate the pharmacodynamics of various chemotherapeutic agents, providing insights into the mechanisms of action and resistance in colorectal cancer therapy. Research utilizing the COLO-205 line has contributed significantly to understanding the biological behaviors typical of colorectal adenocarcinomas, including cellular proliferation, differentiation, and interaction with anticancer drugs.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.