D283Med

The D283Med cell line is a human medulloblastoma cell line that was derived from the cerebellum of a 6-year-old male. Medulloblastoma is a type of primitive neuroectodermal tumor that primarily affects children and is located in the cerebellum, the part of the brain responsible for motor control and coordination. D283Med cells are widely used in oncological research, particularly in studies focused on the biology and pharmacology of medulloblastomas.

This cell line exhibits an adherent growth pattern and has been used extensively to explore the molecular pathways involved in medulloblastoma pathogenesis, such as the Sonic Hedgehog (SHH) and WNT signaling pathways, which are known to play significant roles in the development and progression of these tumors. Researchers utilize the D283Med line to assess therapeutic efficacy and resistance, study gene expression profiles, and explore novel therapeutic targets. The line's robust growth and typical medulloblastoma genetic features make it a valuable model for preclinical studies aimed at understanding tumor biology and testing anticancer drugs.

Furthermore, D283Med cells are utilized in genetic studies to understand the impact of mutations and to assess mechanisms of metastasis and recurrence in medulloblastoma. They provide a crucial tool for the investigation of oncogenic processes at the cellular level, thereby contributing significantly to the development of targeted therapies for this aggressive pediatric brain tumor.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.