K562-GFP

K-562-GFP cells are a genetically modified variant of the human chronic myelogenous leukemia (CML) cell line K-562, originally derived from the peripheral blood of an adult patient in blast crisis. The parental K-562 line is characterized by the presence of the Philadelphia chromosome, resulting in the BCR-ABL fusion protein with constitutive tyrosine kinase activity, which drives uncontrolled proliferation and survival. K-562 cells exhibit erythroleukemia features and can be induced to undergo differentiation along erythroid, megakaryocytic, or monocytic lineages under specific experimental conditions, making them a versatile model for studying hematopoietic differentiation and leukemia biology.

The introduction of green fluorescent protein (GFP) into K-562 cells enables real-time visualization and tracking of leukemic cell behavior in vitro and in vivo. K-562-GFP cells are widely used in assays involving cell proliferation, migration, and drug response, as well as in co-culture systems to study interactions with stromal or immune cells. The fluorescent labeling facilitates applications such as flow cytometry, live-cell imaging, and high-throughput screening.

Genetic modification: Stably modified by replication-incompetent lentiviral transduction to express the ZsGreen1 green fluorescent protein reporter; maintained as a polyclonal population under puromycin selection (1–5 µg/mL). S1/BSL-1 containment.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.