NCI-H2444

NCI-H2444 is a human non-small cell lung cancer (NSCLC) cell line classified within the lung adenocarcinoma spectrum. It was established from a lung tumor specimen obtained from an adult patient and represents an epithelial malignancy of pulmonary origin. As part of large-scale pharmacogenomic and multi-omics characterization efforts, NCI-H2444 has been molecularly profiled alongside extensive panels of human cancer cell lines, enabling integration of genomic, transcriptomic, and pharmacological response data.

In comprehensive drug sensitivity studies of over 1,000 cancer cell lines screened against hundreds of anti-cancer compounds, lung cancer models such as NCI-H2444 have been used to correlate oncogenic alterations with therapeutic vulnerabilities :contentReference[oaicite:0]{index=0}. These analyses incorporate somatic mutation profiles, copy number alterations, DNA methylation patterns, and gene expression data to define clinically relevant cancer functional events and associate them with differential drug response. Such datasets allow NCI-H2444 to be positioned within lineage-specific and mutation-driven sensitivity clusters, supporting its application in biomarker discovery and targeted therapy evaluation.

Proteomic profiling efforts across hundreds of human cancer cell lines have further expanded the molecular annotation framework applicable to models such as NCI-H2444 :contentReference[oaicite:1]{index=1}. High-resolution mass spectrometry-based quantification of thousands of proteins enables integration of proteome-level measurements with transcriptomic and pharmacological datasets. This systems-level characterization facilitates the identification of protein biomarkers predictive of drug response and supports mechanistic studies of pathway activation, post-transcriptional regulation, and therapeutic resistance in lung adenocarcinoma models.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.