SW626

SW626 is a human ovarian cancer cell line established from an adult patient with serous cystadenocarcinoma of the ovary. It has been widely utilized as a model for epithelial ovarian cancer (EOC), particularly for studying tumor biology, drug response, and molecular heterogeneity in high-grade serous carcinoma. Histologically, the SW626 cell line retains characteristics consistent with its serous adenocarcinoma origin and exhibits tumorigenic potential when xenografted into immunocompromised mice, producing solid tumors that recapitulate features of the primary neoplasm.

Genomic profiling of SW626 reveals common alterations frequently observed in ovarian cancers, including disruptions in key regulatory pathways such as TP53 and PI3K/AKT. Molecular analyses have shown that SW626 carries chromosomal aberrations and gene expression patterns representative of high-grade serous ovarian cancer, making it a relevant model for investigating oncogenic signaling, therapeutic vulnerabilities, and resistance mechanisms. The cell line has been included in large-scale cancer genomics projects, where it contributes to drug screening platforms and comparative studies with other ovarian cancer models, helping to define molecular subtypes and inform precision oncology approaches.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.