OVCAR3

OVCAR-3 cells are a human ovarian cancer cell line established from the malignant ascites of a 60-year-old Caucasian female patient with progressive adenocarcinoma of the ovary, refractory to treatment with cyclophosphamide, adriamycin, and cisplatin. Ovcar 3 cells are used in a wide range of studies including drug resistance, particularly those involving DNA damage response biomarkers, homologous recombination repair, and the overall cell cycle dynamics, cancer cell biology, and gene expression studies.

OVCAR-3 cells are epithelial in morphology and have been characterized by their high in vitro growth potential and their ability to form tumors in immunodeficient mice. These cells express several markers characteristic of ovarian carcinoma and have been utilized extensively to study the biology of ovarian cancer.

OVCAR-3 cells are known to have a complex karyotype, with numerous chromosomal abnormalities that are typical of high-grade serous ovarian carcinomas. They are estrogen receptor-positive, which is relatively rare among ovarian cancer cell lines, and this feature is exploited in studies focusing on hormonal influences on ovarian cancer progression and treatment.

In summary, the OVCAR3 cell line stands as a cornerstone in ovarian cancer research, offering a robust model for studying the complex interplay between hormonal influences, drug resistance, and the genetic underpinnings of high-grade ovarian serous adenocarcinoma.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.