SNU-C5

The SNU-C5 cell line is a human gastric carcinoma model established from an adult patient with advanced gastric adenocarcinoma. Derived from a primary tumor specimen, SNU-C5 exhibits epithelial morphology and is part of a broader panel of Korean gastric cancer cell lines developed to represent different histological subtypes and molecular profiles found in East Asian gastric cancers. It provides a valuable model for studying the biology of gastric adenocarcinoma and has been widely used in molecular and pharmacogenomic studies.

Multi-omics profiling, including data from projects like the Cancer Cell Line Encyclopedia (CCLE) and the Genomics of Drug Sensitivity in Cancer (GDSC), has provided a detailed view of SNU-C5’s genetic and pharmacological landscape. The cell line demonstrates common alterations associated with gastric cancer, including mutations in TP53 and alterations in pathways such as PI3K/AKT and RTK signaling. Its inclusion in drug sensitivity screening platforms has allowed researchers to identify associations between genomic features and drug responses, enabling preclinical assessment of targeted therapies. Overall, SNU-C5 serves as a reliable in vitro model for exploring therapeutic vulnerabilities and molecular mechanisms in gastric carcinoma.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.