SNU-C5

SNU-C5 细胞系是一种人类胃癌模型，由一名晚期胃腺癌成年患者建立。SNU-C5 源自原发肿瘤标本，具有上皮形态，是韩国胃癌细胞系的一部分，该细胞系代表了东亚胃癌的不同组织学亚型和分子特征。它为研究胃腺癌生物学提供了一个有价值的模型，并已被广泛用于分子和药物基因组研究。

多组学分析，包括癌症细胞系百科全书（CCLE）和癌症药物敏感性基因组学（GDSC）等项目的数据，提供了SNU-C5基因和药理学图谱的详细视图。该细胞系表现出与胃癌相关的常见改变，包括 TP53 突变和 PI3K/AKT 和 RTK 信号转导等通路的改变。将其纳入药物敏感性筛选平台使研究人员能够确定基因组特征与药物反应之间的关联，从而对靶向疗法进行临床前评估。总之，SNU-C5 是探索胃癌治疗弱点和分子机制的可靠体外模型。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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