Beta-TC-6

Beta-TC-6 細胞是一種源自小鼠胰島素瘤組織的細胞系。這些細胞對於針對糖尿病及胰島素訊號傳導的科學研究至關重要。

Beta-TC-6 細胞源自轉基因小鼠，攜帶一個由 SV40 早期區域組成且受大鼠胰島素基因啟動子調控的假基因構建體。此遺傳組成使其能根據血糖水平分泌胰島素。

這些細胞呈現上皮細胞形態，主要存在於胰臟組織中。除了分泌胰島素外，這些細胞還含有少量胰高血糖素和生長抑素。Beta-TC-6 細胞的黏附特性，使其在實驗與檢測過程中便於培養與操作。

Beta-TC-6 細胞為糖尿病及胰島素訊號傳導的科學研究提供了寶貴的工具。其獨特的遺傳組成、胰島素分泌能力及黏附特性，使其成為研究葡萄糖調節與胰臟功能相關複雜過程的理想選擇。

37°C，5% CO_₂，加濕環境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

透過基於 PCR 的檢測及基於發光原理的支原體檢測方法，排除支原體污染的可能性。

為確保無細菌、真菌或酵母菌污染，每日均會對細胞培養物進行目視檢查。

若需長期保存，請將試管置於溫度約為 −150 至 −196 °C 的氣相液氮中。僅在轉移至液氮之前，可將試管暫時存放於 −80 °C 環境中，作為短期過渡步驟。

冷凍保存的細胞系會以乾冰冷藏，並裝於經驗證的隔熱包裝中，包裝內含有足夠的冷媒，以確保在運輸過程中溫度維持在約 −78 °C。收到貨物後，請立即檢查容器，並盡快將試管轉移至適當的儲存場所。

在 Cytion，我們對產品的品質與存活率提供保障。 我們保證，自本公司設施出貨後 60 天內，我們的細胞在培養開始時仍具存活能力。 我們保證所有材料均符合 分析證明書及／或產品說明書中所載的規格。

詳情請參閱我們的 《保固聲明》。