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UM-SCC-128
Tissue
頭頸部、口腔
Disease
頭頸部鱗狀細胞癌(HNSCC)
Metastatic site
原發性腫瘤部位(頭頸部)

Documents

UM-SCC-128

Cell line · Product number 305726

UM-SCC-128 is a human HNSCC cell line from the University of Michigan UM-SCC panel, growing as an adherent monolayer with epithelial-like morphology. It i...

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01General information

Description

UM-SCC-128 是來自密西根大學 UM-SCC 細胞庫的人類頭頸部鱗狀細胞癌(HNSCC)細胞系,呈黏附性單層生長,具有上皮樣形態。該細胞系屬於編號較高的 UM-SCC 系列,可作為頭頸部鱗狀細胞癌(HNSCC)臨床前比較研究的模型。

適用於頭頸部鱗狀細胞癌(HNSCC)研究、藥物敏感性與抗藥性研究、放射線反應研究,以及 UM-SCC 多株系面板的比較實驗。適合用於藥理篩選及異種移植模型。

Tissue
頭頸部、口腔
Disease
頭頸部鱗狀細胞癌(HNSCC)
Metastatic site
原發性腫瘤部位(頭頸部)
Applications
頭頸部鱗狀細胞癌(HNSCC)研究;藥物敏感性與抗藥性;放射治療反應;密西根大學(UM)鱗狀細胞癌(SCC)研究小組;異種移植模型

02Classification

Organism
Human
Sex
Unspecified
Growth properties
Adherent
GMO status
Non-GMO
GMO status details
No genetic modification; wildtype HNSCC cell line
Biosafety level
1

03Characteristics

Morphology
上皮樣
Cell type
上皮細胞
Doubling time
約 24 至 48 小時

04Identifiers

Citation
UM-SCC-128(Cytion 型號 305726)
NCBI TaxID
9606
Cellosaurus
Not assigned

05Handling and culture

Culture medium
DMEM,含 4.5 g/L 葡萄糖、4 mM L-谷氨醯胺、3.7 g/L NaHCO₃ 及 1.0 mM 丙酮酸鈉(Cytion 產品編號 820300a)
Incubation atmosphere

37°C,5% CO₂,加濕環境。

Supplements
在培養基中加入 10% 的胎牛血清(FBS)及 1% 的非必需胺基酸(NEAA)
Dissociation reagent
Accutase
Subculturing
移除培養基,用不含鈣和鎂的PBS清洗,加入Accutase覆蓋,於室溫下孵育 8–10 分鐘,重新懸浮於培養基中,以 300×g 離心 3 分鐘,棄去上清液,再將細胞接種於新鮮培養基中。
Split ratio
1 至 5
Seeding density
1 至 3 × 10⁴ 個細胞/cm²
Fluid renewal
每 2 至 3 天
Thawing and culturing
  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

  7. Follow the procedure described under Post-Thaw Recovery

06Quality control and cryopreservation

Freeze medium
作為冷凍保存培養基,我們使用完全生長培養基加上 10% 的二甲基亞砜(DMSO),以確保解凍後的細胞存活率達到適當水準。
Storage conditions

若需長期保存,請將試管置於溫度約為 −150 至 −196 °C 的氣相液氮中。僅在轉移至液氮之前,可將試管暫時存放於 −80 °C 環境中,作為短期過渡步驟。

Shipping conditions

冷凍保存的細胞系會以乾冰冷藏,並裝於經驗證的隔熱包裝中,包裝內含有足夠的冷媒,以確保在運輸過程中溫度維持在約 −78 °C。收到貨物後,請立即檢查容器,並盡快將試管轉移至適當的儲存場所。

07Donor

Donor age
年齡不詳
Ethnicity
未指定族裔