HMy2.CIR

HMy2.CIR细胞系是通过伽马辐照和随后的选择，使HMy.2 B淋巴母细胞系失去HLA I类抗原表达而培育出来的。该亲本细胞系是源自 ARH-77 细胞系的快速生长突变体。HMy2.CIR 细胞作为转染 I 类主要组织相容性抗原基因的宿主特别有价值，为研究抗原呈递和免疫反应机制提供了一个多功能平台。

众所周知，HMy2.CIR 最终来源的 ARH-77 细胞系对 Epstein-Barr 核抗原（ENA+）和 Epstein-Barr 病毒荚膜抗原（EBVCA+）呈阳性反应。因此，HMy2.CIR 细胞系也被推测为 EBNA 阳性。该细胞系的特点是表达少量 HLA Cw4，但不表达 HLA A 或 B 基因座产物。这种独特的抗原表达谱使 HMy2.CIR 细胞成为免疫学研究的有用模型，特别是在研究 HLA I 类受限抗原的处理和递呈方面。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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