HROGas03

HROGas03 细胞系来源于一名成年女性患者的胃腺癌。胃腺癌是一种常见的胃癌，来自胃黏膜的腺上皮细胞，通常预后不良。作为一种模型，HROGas03 为研究胃腺癌的发生、发展和耐药性的分子途径提供了宝贵的资源。供体的年龄相关性，如潜在的基因组不稳定性和肿瘤微环境的改变，可以为研究老年人的癌症生物学提供独特的见解。

这种细胞系使研究人员能够探索驱动胃癌的关键分子机制，如肿瘤抑制基因（如 TP53）的突变、细胞周期的改变和信号通路的失调，包括 Wnt、MAPK 和 PI3K/AKT 通路。这些通路往往与胃癌细胞的生存、增殖和转移潜能有关。HROGas03 细胞系还可用于评估靶向疗法、化疗药物或联合疗法的疗效，从而有可能改进胃癌患者的治疗策略，尤其是老年人群。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

有关详细信息，请参阅我们的 <a href="" /Who-we-are/Warranty/"" hreflang=""x-default"">保修声明 。