MA-CLS-2 细胞

MA-CLS-2 细胞系是根据一名女性乳腺导管癌患者的胸腔积液建立的。该细胞系来源于人类乳腺肿瘤，特别代表了通常与癌症晚期相关的胸膜转移。原始肿瘤被归类为 pT1 NO GII，表明原发肿瘤大小有限（T1），无区域淋巴结转移（N0），并被分级为中度分化（GII）。这些特征表明，肿瘤处于相对早期，但已经扩散到胸膜腔，这种并发症对患者的预后有很大影响。

MA-CLS-2 对研究乳腺癌的转移过程特别有价值，尤其是涉及胸腔积液的转移过程，它可以帮助人们深入了解肿瘤扩散的机制和潜在的治疗靶点。该细胞系为研究转移性乳腺癌细胞与胸膜环境之间的相互作用提供了一个模型，有助于研究旨在预防或治疗转移性疾病的新型干预措施。作为一种来源于导管癌的胸膜转移模型，MA-CLS-2 还可以用于研究转移性乳腺癌的药物反应。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

有关详细信息，请参阅我们的 <a href="" /Who-we-are/Warranty/"" hreflang=""x-default"">保修声明 。