SHP-77

SHP-77 细胞系是一种人类小细胞肺癌（SCLC）模型。它来源于原发性肺肿瘤，被广泛用于癌症研究，特别是肺癌生物学和药物开发研究。SHP-77 细胞具有 SCLC 的典型特征，包括在异种移植模型中的快速生长和高致瘤性。这种细胞系以其在血清补充培养基中的增殖能力而闻名，已被用于各种实验装置，如研究致癌信号通路和对化疗药物的治疗反应。

SHP-77细胞是癌症细胞系百科全书（CCLE）的一部分，该资源使研究人员能够将基因图谱与药物敏感性联系起来。SHP-77的基因组图谱揭示了关键癌基因和肿瘤抑制因子的突变和改变，为研究SCLC发病的分子机制提供了一个平台。该细胞系还被纳入了药物筛选研究，使人们对其药理弱点有了深入了解，并有助于鉴定对肺癌有治疗潜力的化合物。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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