SNU-668

SNU-668 细胞系是一种人类胃癌模型，最初来源于分化较差的胃腺癌组织。该细胞系已被广泛用于胃癌发病机制、信号转导机制和药物反应性的研究。基因组特征分析显示，SNU-668 携带弥漫型胃癌中常见的突变和染色体畸变。值得注意的是，它显示出关键致癌通路的改变，如 TP53 突变和 PI3K/AKT 信号的可能激活，这可能是其致瘤特性和耐药性的原因。

SNU-668 还被纳入了癌症细胞系百科全书（CCLE）等综合多组学分析项目，在这些项目中对其进行了转录组、基因组、甲基化和蛋白质组特征评估。该细胞系表现出独特的 DNA 甲基化模式和全局组蛋白修饰特征，可能在基因表达的表观遗传调控中发挥作用。此外，对依赖性图谱的分析表明，细胞系特有的脆弱性可为弥漫性胃癌的靶向治疗策略提供依据。作为一种具有亚洲种族背景的胃癌模型，SNU-668 仍然是分子引导疗法临床前评估的重要工具。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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