Buffer en supplementen

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Antibiotic/Antimycotic Solution (100x)

Volume: 100 ml Storage: ≤-15°C Sterility: Sterile-filtered Antibiotic/Antimycotic Solution (100x) is a sterile, ready-to-use concentrate designed to reduce microbial contamination risks in cell culture and related laboratory applications. This 100x solution contains a well-established combination of penicillin, streptomycin, and amphotericin B—providing broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts, and filamentous fungi. The formulation is suitable for use in eukaryotic cell cultures, bacterial media, and other contamination-sensitive systems, supporting clean and consistent lab operations. Application and Benefits Optimized for routine research protocols, this solution is widely used to maintain aseptic conditions in cell culture workflows. It offers reliable performance in contamination-sensitive environments, helping researchers reduce the risk of microbial overgrowth without compromising cell health or experimental reproducibility. The sterile-filtered formulation eliminates the need for additional solubilization steps, supporting streamlined media preparation and reducing variability in daily lab procedures. Usage and Compatibility To achieve standard working concentrations, dilute the solution 1:100 into your complete culture medium. The product is compatible with a broad range of mammalian cell lines and basal media. With consistent stock availability, researchers benefit from dependable supply continuity and simplified logistics planning. The solution should be stored at ≤ –15 °C and protected from repeated freeze-thaw cycles to maintain stability. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€45.00

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D-Glucose Solution (Dextrose Solution), 250 g/L

Volume: 50 ml Storage: +2°C to +8°C Sterility: Sterile-filtered D-(+)-Glucose Solution (Dextrose Solution) is a sterile, ready-to-use supplement containing the naturally occurring sugar D-(+)-glucose, a central component of cellular metabolism. Glucose is involved in essential biological processes such as energy production, glycosylation, and the formation of glycans that contribute to cellular structure and function. Application and Benefits This glucose solution is widely used as a supplement in cell culture media and in numerous cellular and molecular biology applications. As a primary carbon and energy source, glucose supports cell growth, proliferation, and metabolic activity. Its involvement in biosynthetic pathways also makes it critical for maintaining normal cellular physiology and experimental consistency. Formulation and Usage The solution is supplied at a high concentration of 250 g/L glucose, allowing flexible dilution into culture media according to experimental requirements. It is sterile-filtered to ensure suitability for contamination-sensitive applications. Store at +2°C to +8°C and handle aseptically to maintain product quality and stability. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€32.00

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500 ml bottle Fetal Bovine Serum South America Origin (FBS)

FBS Zuid-Amerika 500 ml

Dit steriel gefilterde foetaal runderserum (FBS), afkomstig van Zuid-Amerikaanse runderen, zorgt voor consistente resultaten bij celkweek. Voorafgaand aan vrijgave wordt elke partij getest op steriliteit, mycoplasma en runder virussen. Bovendien wordt elke partij beoordeeld om te controleren of deze de groei van HEK293- en CHO-cellen ondersteunt. Het serum is verkrijgbaar in verpakkingen van 50 ml en 500 ml, en op verzoek kan een partijspecifiek analysecertificaat (CoA) worden verstrekt.

€310.00

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50 ml bottle Fetal Bovine Serum South America Origin (FBS)

FBS South Origin 50 ml

€32.00

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FBS US 500 ml

Dit steriel gefilterde foetaal runderserum (FBS), afkomstig uit de Verenigde Staten, biedt een consistente basis voor celkweektoepassingen. Elke batch wordt onderworpen aan een kwaliteitscontrole op steriliteit, mycoplasma en runder virussen. Bovendien wordt elke partij biologisch gevalideerd om te bevestigen dat deze de groei van CHO- en HEK293-cellijnen ondersteunt. Het serum wordt verpakt in verpakkingen van 50 ml en 500 ml. Partijspecifieke analysecertificaten (CoA) zijn op verzoek verkrijgbaar.

€506.00

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500 ml bottle Fetal Bovine Serum US Origin (FBS)

FBS US Origin 50 ml

Dit steriel gefilterde foetaal runderserum (FBS), afkomstig uit de Verenigde Staten, biedt een consistente basis voor celkweektoepassingen. Elke batch wordt onderworpen aan een kwaliteitscontrole op steriliteit, mycoplasma en runder virussen. Bovendien wordt elke partij biologisch gevalideerd om te bevestigen dat het de groei van CHO- en HEK293-cellijnen ondersteunt. Het serum wordt verpakt in verpakkingen van 50 ml en 500 ml. Partijspecifieke analysecertificaten (CoA) zijn op verzoek verkrijgbaar.

€52.00

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HEPES Buffer Solution (1M)

Volume: 100 ml Storage: +2°C to +8°C Sterility: Sterile-filtered HEPES Buffer Solution (1 M), also known as N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid, is a zwitterionic organic buffering agent widely used in cell culture media. It is designed to maintain stable pH conditions in the physiological range of 6.7 to 8.6, supporting optimal cellular function during in vitro applications. Application and Benefits HEPES provides reliable buffering capacity in cell culture systems, particularly when cells are handled outside a CO₂ incubator. The addition of 10–25 mM HEPES to culture media offers enhanced pH stability during extended manipulation periods, helping to maintain consistent experimental conditions. This buffer is membrane-impermeable, has minimal interference with biochemical reactions, and demonstrates strong chemical and enzymatic stability. These properties make it suitable for a wide range of cell culture and biochemical applications. Formulation and Usage The solution is supplied as a 1 M concentrate prepared in cell culture–grade water and is sterile-filtered for use in contamination-sensitive environments. It can be diluted to the desired working concentration depending on application requirements. Store at +2°C to +8°C and handle under aseptic conditions to preserve product integrity. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€52.00

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Non-essential Amino Acids (100x)

Volume: 100 ml Storage: +2°C to +8°C Sterility: Sterile-filtered MEM Non-Essential Amino Acids (100x) is a sterile supplement designed to enhance cell growth and viability in mammalian cell culture systems. The formulation corresponds to a 100x concentrate of the non-essential amino acids found in standard Minimum Essential Medium (MEM), allowing for direct supplementation of basal media with minimal preparation. Application and Benefits This supplement provides an additional amino acid pool for rapidly proliferating cells or for cell lines that have lost the capacity to synthesize non-essential amino acids de novo. By alleviating the metabolic burden of biosynthesis, it supports improved growth kinetics, prolonged viability, and greater experimental consistency—especially in nutrient-sensitive or high-density cultures. Composition and Use The solution includes glycine, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, L-proline, and L-serine. It is compatible with MEM and most other standard media. To use, dilute 1:100 into the final culture medium. This product is sterile-filtered and ready to use without additional handling steps. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€30.00

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PBS

Fosfaatgebufferde zoutoplossing (PBS) is een veelgebruikte bufferoplossing in biologisch en chemisch onderzoek. Het speelt een cruciale rol bij het handhaven van de pH-balans en de osmolariteit tijdens diverse experimentele procedures, waaronder weefselverwerking en celkweek. Onze PBS-oplossing is zorgvuldig samengesteld uit ingrediënten van hoge zuiverheid om stabiliteit en betrouwbaarheid bij elk experiment te garanderen. De osmolariteit en ionenconcentraties van onze PBS komen nauw overeen met die van het menselijk lichaam, waardoor de oplossing isotoon en niet-toxisch is voor de meeste cellen. Samenstelling van onze PBS-oplossing Onze PBS-oplossing is een mengsel van fosfaatbuffers van ultrazuivere kwaliteit en zoutoplossingen, waarvan de pH is aangepast. Bij een werkconcentratie van 1X bevat deze: • 8000 mg/L natriumchloride (NaCl) • 200 mg/L kaliumchloride (KCl) • 1150 mg/L watervrij dibasisch natriumfosfaat (Na₂HPO₄) • 200 mg/L watervrij monobasisch kaliumfosfaat (KH₂PO₄) Deze samenstelling zorgt voor een optimale pH-waarde en ionenbalans, geschikt voor een breed scala aan biologische toepassingen. Toepassingen van onze PBS-oplossing Onze PBS-oplossing is ideaal voor diverse toepassingen in biologisch onderzoek. Dankzij de isotone en niet-toxische eigenschappen is deze geschikt voor het verdunnen van stoffen en het spoelen van celcultuurvaten. PBS-oplossingen die EDTA bevatten, zijn effectief voor het losmaken van aan elkaar gehechte en samengeklonterde cellen. Tweewaardige metalen zoals zink mogen echter niet aan PBS worden toegevoegd, aangezien dit neerslag kan veroorzaken. In dergelijke gevallen worden Good's-buffers aanbevolen. Bovendien is onze PBS-oplossing een geschikt alternatief voor viraal transportmedium voor het transport en de opslag van RNA-virussen, waaronder SARS-CoV-2. Kwaliteitscontrole • Steriel gefilterd Opslag en houdbaarheid • Bewaren bij +2 °C tot +25 °C, beschermd tegen licht. • Na opening bewaren bij 2 °C tot 25 °C en binnen 24 maanden gebruiken. Verzendvoorwaarden • Omgevingstemperatuur Bewaring • Bewaar gekoeld bij +2 °C tot +8 °C in het donker. Vermijd bevriezing en herhaaldelijk opwarmen tot +37 °C, aangezien dit de productkwaliteit vermindert. • Verwarm het medium niet tot boven 37 °C en gebruik geen ongecontroleerde warmtebronnen zoals magnetrons. • Als slechts een deel van het medium wordt gebruikt, neem dan de benodigde hoeveelheid eruit en breng deze vóór gebruik op kamertemperatuur. Samenstelling Categorie Bestanddelen Concentratie (mg/l) Zouten Kaliumchloride 200 Monobasisch kaliumfosfaat, watervrij 200 Natriumchloride 8000 Dibasisch natriumfosfaat, watervrij 1150

€20.00

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Sodium Pyruvate Solution (100 mM)

Volume: 100 ml Storage: +2°C to +8°C Sterility: Sterile-filtered Sodium Pyruvate Solution (100 mM) is a sterile, ready-to-use supplement designed to provide an additional, easily accessible energy source for cell culture media. Sodium pyruvate plays a key role in cellular energy metabolism and supports the growth of metabolically active and rapidly proliferating cells, such as tumor cells. Supplementation can enhance cell viability and help maintain metabolic stability in culture systems. Application and Benefits This solution is widely used in routine cell culture to enrich media with pyruvate and promote optimal growth conditions. It supports ATP production, may help reduce oxidative stress, and contributes to improved metabolic performance of cultured cells. Manufactured in cell culture–grade water and sterile-filtered, the product ensures consistent quality and reproducibility in research workflows. Usage and Compatibility The recommended final concentration for most cell culture applications is 1 mM sodium pyruvate, achieved by diluting the 100 mM stock solution 1:100 into complete culture medium. The solution is compatible with a wide range of basal media and mammalian cell lines. Store at +2°C to +8°C and protect from contamination to maintain product stability. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€18.00

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Stable Glutamin (200mM), L-Alanyl-L-Glutamine Solution

Volume: 100 ml Storage: ≤ –15°C Sterility: Sterile-filtered Stable Glutamine Solution (L-Alanyl-L-Glutamine, 200 mM) is a highly stable dipeptide form of L-glutamine designed as a direct replacement for conventional L-glutamine in cell culture media. L-glutamine is an essential amino acid and a major energy source for cultured cells, playing a critical role in cell growth, metabolism, and protein synthesis. Application and Benefits In standard liquid media, L-glutamine degrades relatively quickly at 37°C, leading to the formation of toxic byproducts such as ammonium ions that can negatively affect cell viability and experimental outcomes. Stable glutamine overcomes this limitation by providing a non-degrading dipeptide form that remains intact in culture conditions. Cells enzymatically cleave the dipeptide bond to release L-glutamine as needed, ensuring a continuous fresh supply while preventing the accumulation of harmful waste products. This makes the solution particularly advantageous for long-term cell cultures and high-density growth systems. Formulation and Usage The L-Alanyl-L-Glutamine solution is prepared in cell culture–grade water at a concentration of 200 mM and is sterile-filtered for contamination-sensitive applications. It can be diluted directly into complete media according to experimental requirements. Store at ≤ –15°C and avoid repeated freeze-thaw cycles to maintain product stability. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€52.00

excl. btw en verzendkosten