Langsung ke isi

Buffer dan Suplemen

11 produk

Antibiotic/Antimycotic Solution (100x)

Volume: 100 ml Storage: ≤-15°C Sterility: Sterile-filtered Antibiotic/Antimycotic Solution (100x) is a sterile, ready-to-use concentrate designed to reduce microbial contamination risks in cell culture and related laboratory applications. This 100x solution contains a well-established combination of penicillin, streptomycin, and amphotericin B—providing broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts, and filamentous fungi. The formulation is suitable for use in eukaryotic cell cultures, bacterial media, and other contamination-sensitive systems, supporting clean and consistent lab operations. Application and Benefits Optimized for routine research protocols, this solution is widely used to maintain aseptic conditions in cell culture workflows. It offers reliable performance in contamination-sensitive environments, helping researchers reduce the risk of microbial overgrowth without compromising cell health or experimental reproducibility. The sterile-filtered formulation eliminates the need for additional solubilization steps, supporting streamlined media preparation and reducing variability in daily lab procedures. Usage and Compatibility To achieve standard working concentrations, dilute the solution 1:100 into your complete culture medium. The product is compatible with a broad range of mammalian cell lines and basal media. With consistent stock availability, researchers benefit from dependable supply continuity and simplified logistics planning. The solution should be stored at ≤ –15 °C and protected from repeated freeze-thaw cycles to maintain stability. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€45.00

belum termasuk pajak dan ongkos kirim

HEPES Buffer Solution (1M)

Volume: 100 ml Storage: +2°C to +8°C Sterility: Sterile-filtered HEPES Buffer Solution (1 M), also known as N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid, is a zwitterionic organic buffering agent widely used in cell culture media. It is designed to maintain stable pH conditions in the physiological range of 6.7 to 8.6, supporting optimal cellular function during in vitro applications. Application and Benefits HEPES provides reliable buffering capacity in cell culture systems, particularly when cells are handled outside a CO₂ incubator. The addition of 10–25 mM HEPES to culture media offers enhanced pH stability during extended manipulation periods, helping to maintain consistent experimental conditions. This buffer is membrane-impermeable, has minimal interference with biochemical reactions, and demonstrates strong chemical and enzymatic stability. These properties make it suitable for a wide range of cell culture and biochemical applications. Formulation and Usage The solution is supplied as a 1 M concentrate prepared in cell culture–grade water and is sterile-filtered for use in contamination-sensitive environments. It can be diluted to the desired working concentration depending on application requirements. Store at +2°C to +8°C and handle under aseptic conditions to preserve product integrity. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€52.00

belum termasuk pajak dan ongkos kirim

PBS

Larutan garam yang dibuffer dengan fosfat (PBS) merupakan larutan penyangga yang banyak digunakan dalam penelitian biologi dan kimia. Larutan ini memainkan peran penting dalam menjaga keseimbangan pH dan osmolaritas selama berbagai prosedur eksperimental, termasuk pengolahan jaringan dan kultur sel. Larutan PBS kami diformulasikan dengan cermat menggunakan bahan-bahan dengan kemurnian tinggi untuk memastikan stabilitas dan keandalan dalam setiap eksperimen. Osmolaritas dan konsentrasi ion PBS kami sangat mirip dengan yang terdapat dalam tubuh manusia, sehingga bersifat isotonik dan tidak beracun bagi sebagian besar sel. Komposisi Larutan PBS Kami Larutan PBS kami merupakan campuran larutan penyangga fosfat dan larutan garam dengan tingkat kemurnian ultra tinggi yang telah disesuaikan pH-nya. Pada konsentrasi kerja 1X, larutan ini mengandung: • 8000 mg/L Natrium klorida (NaCl) • 200 mg/L Kalium klorida (KCl) • 1.150 mg/L natrium fosfat dibasik anhidrat (Na₂HPO₄) • 200 mg/L Kalium fosfat monobasik anhidrat (KH₂PO₄) Komposisi ini memastikan pH dan keseimbangan ionik yang optimal, sehingga cocok untuk berbagai aplikasi biologis. Aplikasi Larutan PBS Kami Larutan PBS kami sangat ideal untuk berbagai aplikasi dalam penelitian biologi. Sifatnya yang isotonik dan tidak beracun membuatnya cocok untuk pengenceran zat dan pembilasan wadah sel. Larutan PBS yang mengandung EDTA efektif untuk melepaskan sel-sel yang menempel dan menggumpal. Namun, logam divalen seperti seng tidak boleh ditambahkan ke dalam PBS, karena hal ini dapat menyebabkan pengendapan. Dalam kasus seperti itu, disarankan untuk menggunakan buffer Good. Selain itu, larutan PBS kami merupakan alternatif yang dapat diterima sebagai media transportasi virus untuk pengangkutan dan penyimpanan virus RNA, termasuk SARS-CoV-2. Pengendalian Kualitas • Disaring secara steril Penyimpanan dan Umur Simpan • Simpan pada suhu +2°C hingga +25°C, terlindung dari cahaya. • Setelah dibuka, simpan pada suhu 2°C hingga 25°C dan gunakan dalam waktu 24 bulan. Kondisi Pengiriman • Suhu kamar Perawatan • Simpan dalam lemari pendingin pada suhu +2°C hingga +8°C di tempat gelap. Hindari pembekuan dan pemanasan berulang hingga +37°C, karena hal ini dapat menurunkan kualitas produk. • Jangan memanaskan media melebihi 37°C atau menggunakan sumber panas yang tidak terkendali seperti alat microwave. • Jika hanya sebagian media yang akan digunakan, ambil jumlah yang diperlukan dan panaskan hingga suhu kamar sebelum digunakan. Komposisi Kategori Komponen Konsentrasi (mg/L) Garam Kalium klorida 200 Fosfat kalium monobasik anhidrat 200 Natrium klorida 8000 Natrium fosfat dibasik anhidrat 1150

€20.00

belum termasuk pajak dan ongkos kirim

Stable Glutamin (200mM), L-Alanyl-L-Glutamine Solution

Volume: 100 ml Storage: ≤ –15°C Sterility: Sterile-filtered Stable Glutamine Solution (L-Alanyl-L-Glutamine, 200 mM) is a highly stable dipeptide form of L-glutamine designed as a direct replacement for conventional L-glutamine in cell culture media. L-glutamine is an essential amino acid and a major energy source for cultured cells, playing a critical role in cell growth, metabolism, and protein synthesis. Application and Benefits In standard liquid media, L-glutamine degrades relatively quickly at 37°C, leading to the formation of toxic byproducts such as ammonium ions that can negatively affect cell viability and experimental outcomes. Stable glutamine overcomes this limitation by providing a non-degrading dipeptide form that remains intact in culture conditions. Cells enzymatically cleave the dipeptide bond to release L-glutamine as needed, ensuring a continuous fresh supply while preventing the accumulation of harmful waste products. This makes the solution particularly advantageous for long-term cell cultures and high-density growth systems. Formulation and Usage The L-Alanyl-L-Glutamine solution is prepared in cell culture–grade water at a concentration of 200 mM and is sterile-filtered for contamination-sensitive applications. It can be diluted directly into complete media according to experimental requirements. Store at ≤ –15°C and avoid repeated freeze-thaw cycles to maintain product stability. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€52.00

belum termasuk pajak dan ongkos kirim