Preskoči na vsebino

Pufri in dodatki

11 izdelki

Antibiotic/Antimycotic Solution (100x)

Volume: 100 ml Storage: ≤-15°C Sterility: Sterile-filtered Antibiotic/Antimycotic Solution (100x) is a sterile, ready-to-use concentrate designed to reduce microbial contamination risks in cell culture and related laboratory applications. This 100x solution contains a well-established combination of penicillin, streptomycin, and amphotericin B—providing broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts, and filamentous fungi. The formulation is suitable for use in eukaryotic cell cultures, bacterial media, and other contamination-sensitive systems, supporting clean and consistent lab operations. Application and Benefits Optimized for routine research protocols, this solution is widely used to maintain aseptic conditions in cell culture workflows. It offers reliable performance in contamination-sensitive environments, helping researchers reduce the risk of microbial overgrowth without compromising cell health or experimental reproducibility. The sterile-filtered formulation eliminates the need for additional solubilization steps, supporting streamlined media preparation and reducing variability in daily lab procedures. Usage and Compatibility To achieve standard working concentrations, dilute the solution 1:100 into your complete culture medium. The product is compatible with a broad range of mammalian cell lines and basal media. With consistent stock availability, researchers benefit from dependable supply continuity and simplified logistics planning. The solution should be stored at ≤ –15 °C and protected from repeated freeze-thaw cycles to maintain stability. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€45.00

brez davka in stroškov poštnine

HEPES Buffer Solution (1M)

Volume: 100 ml Storage: +2°C to +8°C Sterility: Sterile-filtered HEPES Buffer Solution (1 M), also known as N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid, is a zwitterionic organic buffering agent widely used in cell culture media. It is designed to maintain stable pH conditions in the physiological range of 6.7 to 8.6, supporting optimal cellular function during in vitro applications. Application and Benefits HEPES provides reliable buffering capacity in cell culture systems, particularly when cells are handled outside a CO₂ incubator. The addition of 10–25 mM HEPES to culture media offers enhanced pH stability during extended manipulation periods, helping to maintain consistent experimental conditions. This buffer is membrane-impermeable, has minimal interference with biochemical reactions, and demonstrates strong chemical and enzymatic stability. These properties make it suitable for a wide range of cell culture and biochemical applications. Formulation and Usage The solution is supplied as a 1 M concentrate prepared in cell culture–grade water and is sterile-filtered for use in contamination-sensitive environments. It can be diluted to the desired working concentration depending on application requirements. Store at +2°C to +8°C and handle under aseptic conditions to preserve product integrity. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€52.00

brez davka in stroškov poštnine

PBS

Fosfatno pufrirana fiziološka raztopina (PBS) je v bioloških in kemijskih raziskavah široko uporabljena pufrska raztopina. Ima ključno vlogo pri ohranjanju ravnovesja pH in osmolaritete med različnimi eksperimentalnimi postopki, vključno z obdelavo tkiv in gojenjem celic. Naša raztopina PBS je skrbno sestavljena iz sestavin visoke čistosti, kar zagotavlja stabilnost in zanesljivost pri vsakem poskusu. Osmolariteta in koncentracije ionov v naši raztopini PBS tesno posnemajo tiste v človeškem telesu, zaradi česar je izotonična in nestrupena za večino celic. Sestava naše PBS-raztopine Naša raztopina PBS je mešanica fosfatnih pufrov in fizioloških raztopin ultračistega razreda z uravnanim pH. Pri delovni koncentraciji 1X vsebuje: • 8000 mg/L natrijevega klorida (NaCl) • 200 mg/L kalijevega klorida (KCl) • 1150 mg/l brezvodnega dibasnega natrijevega fosfata (Na₂HPO₄) • 200 mg/L brezvodnega enobaznega kalijevega fosfata (KH₂PO₄) Ta sestava zagotavlja optimalno vrednost pH in ionsko ravnovesje, primerno za široko paleto bioloških uporab. Uporaba naše PBS-raztopine Naša PBS-raztopina je idealna za različne uporabe v bioloških raziskavah. Zaradi svojih izotoničnih in netoksičnih lastnosti je primerna za redčenje snovi in izpiranje posod za celice. PBS-raztopine, ki vsebujejo EDTA, so učinkovite za ločevanje pritrjenih in zlepljenih celic. Vendar pa se v PBS ne smejo dodajati dvovalentne kovine, kot je cink, saj lahko to povzroči usedanje. V takih primerih priporočamo Goodove pufre. Poleg tega je naša PBS-raztopina sprejemljiva alternativa virusnemu transportnemu mediju za prevoz in shranjevanje RNA-virusov, vključno s SARS-CoV-2. Kontrola kakovosti • Sterilno filtrirana Shranjevanje in rok uporabnosti • Shranjujte pri temperaturi od +2 °C do +25 °C, zaščiteno pred svetlobo. • Po odprtju shranjujte pri temperaturi od 2 °C do 25 °C in porabite v 24 mesecih. Pogoji za pošiljanje • Temperatura okolja Shranjevanje • Hranite v hladilniku pri temperaturi od +2 °C do +8 °C na temnem mestu. Izogibajte se zamrzovanju in pogostemu segrevanju na +37 °C, saj to zmanjša kakovost izdelka. • Medija ne segrevajte nad 37 °C in ne uporabljajte nekontroliranih virov toplote, kot so mikrovalovne pečice. • Če nameravate uporabiti le del gojišča, odvzemite potrebno količino in jo pred uporabo segrejte na sobno temperaturo. Sestava Kategorija Sestavine Koncentracija (mg/l) Soli Kalijev klorid 200 Enobazni brezvodni kalijev fosfat 200 Natrijev klorid 8000 Dibazični natrijev fosfat, brezvodni 1150

€20.00

brez davka in stroškov poštnine

Stable Glutamin (200mM), L-Alanyl-L-Glutamine Solution

Volume: 100 ml Storage: ≤ –15°C Sterility: Sterile-filtered Stable Glutamine Solution (L-Alanyl-L-Glutamine, 200 mM) is a highly stable dipeptide form of L-glutamine designed as a direct replacement for conventional L-glutamine in cell culture media. L-glutamine is an essential amino acid and a major energy source for cultured cells, playing a critical role in cell growth, metabolism, and protein synthesis. Application and Benefits In standard liquid media, L-glutamine degrades relatively quickly at 37°C, leading to the formation of toxic byproducts such as ammonium ions that can negatively affect cell viability and experimental outcomes. Stable glutamine overcomes this limitation by providing a non-degrading dipeptide form that remains intact in culture conditions. Cells enzymatically cleave the dipeptide bond to release L-glutamine as needed, ensuring a continuous fresh supply while preventing the accumulation of harmful waste products. This makes the solution particularly advantageous for long-term cell cultures and high-density growth systems. Formulation and Usage The L-Alanyl-L-Glutamine solution is prepared in cell culture–grade water at a concentration of 200 mM and is sterile-filtered for contamination-sensitive applications. It can be diluted directly into complete media according to experimental requirements. Store at ≤ –15°C and avoid repeated freeze-thaw cycles to maintain product stability. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€52.00

brez davka in stroškov poštnine