Buffer a doplňky

11 výrobky

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Antibiotic/Antimycotic Solution (100x)

Volume: 100 ml Storage: ≤-15°C Sterility: Sterile-filtered Antibiotic/Antimycotic Solution (100x) is a sterile, ready-to-use concentrate designed to reduce microbial contamination risks in cell culture and related laboratory applications. This 100x solution contains a well-established combination of penicillin, streptomycin, and amphotericin B—providing broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts, and filamentous fungi. The formulation is suitable for use in eukaryotic cell cultures, bacterial media, and other contamination-sensitive systems, supporting clean and consistent lab operations. Application and Benefits Optimized for routine research protocols, this solution is widely used to maintain aseptic conditions in cell culture workflows. It offers reliable performance in contamination-sensitive environments, helping researchers reduce the risk of microbial overgrowth without compromising cell health or experimental reproducibility. The sterile-filtered formulation eliminates the need for additional solubilization steps, supporting streamlined media preparation and reducing variability in daily lab procedures. Usage and Compatibility To achieve standard working concentrations, dilute the solution 1:100 into your complete culture medium. The product is compatible with a broad range of mammalian cell lines and basal media. With consistent stock availability, researchers benefit from dependable supply continuity and simplified logistics planning. The solution should be stored at ≤ –15 °C and protected from repeated freeze-thaw cycles to maintain stability. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€45.00

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D-Glucose Solution (Dextrose Solution), 250 g/L

Volume: 50 ml Storage: +2°C to +8°C Sterility: Sterile-filtered D-(+)-Glucose Solution (Dextrose Solution) is a sterile, ready-to-use supplement containing the naturally occurring sugar D-(+)-glucose, a central component of cellular metabolism. Glucose is involved in essential biological processes such as energy production, glycosylation, and the formation of glycans that contribute to cellular structure and function. Application and Benefits This glucose solution is widely used as a supplement in cell culture media and in numerous cellular and molecular biology applications. As a primary carbon and energy source, glucose supports cell growth, proliferation, and metabolic activity. Its involvement in biosynthetic pathways also makes it critical for maintaining normal cellular physiology and experimental consistency. Formulation and Usage The solution is supplied at a high concentration of 250 g/L glucose, allowing flexible dilution into culture media according to experimental requirements. It is sterile-filtered to ensure suitability for contamination-sensitive applications. Store at +2°C to +8°C and handle aseptically to maintain product quality and stability. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€32.00

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500 ml bottle Fetal Bovine Serum South America Origin (FBS)

FBS Jižní Amerika 500 ml

Toto sterilně filtrované fetální hovězí sérum (FBS), získané z jihoamerických skotů, zajišťuje konzistentní výsledky při kultivaci buněk. Před uvolněním na trh je každá šarže testována na sterilitu, mykoplazmy a hovězí viry. Kromě toho je každá šarže vyhodnocena z hlediska schopnosti podporovat růst buněk HEK293 a CHO. Sérum je k dispozici v objemech 50 ml a 500 ml a na požádání lze poskytnout certifikát analýzy (CoA) pro konkrétní šarži.

€310.00

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50 ml bottle Fetal Bovine Serum South America Origin (FBS)

FBS South Origin 50 ml

€32.00

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FBS US 500 ml

Toto sterilně filtrované fetální hovězí sérum (FBS) pocházející ze Spojených států poskytuje konzistentní základ pro aplikace v oblasti buněčných kultur. Každá šarže prochází kontrolou kvality zaměřenou na sterilitu, mykoplazmy a hovězí viry. Každá šarže je navíc biologicky validována, aby byla potvrzena její schopnost podporovat růst buněčných linií CHO a HEK293. Sérum je baleno v objemech 50 ml a 500 ml. Certifikáty analýzy (CoA) pro jednotlivé šarže jsou k dispozici na vyžádání.

€506.00

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500 ml bottle Fetal Bovine Serum US Origin (FBS)

FBS US Origin 50 ml

€52.00

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HEPES Buffer Solution (1M)

Volume: 100 ml Storage: +2°C to +8°C Sterility: Sterile-filtered HEPES Buffer Solution (1 M), also known as N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid, is a zwitterionic organic buffering agent widely used in cell culture media. It is designed to maintain stable pH conditions in the physiological range of 6.7 to 8.6, supporting optimal cellular function during in vitro applications. Application and Benefits HEPES provides reliable buffering capacity in cell culture systems, particularly when cells are handled outside a CO₂ incubator. The addition of 10–25 mM HEPES to culture media offers enhanced pH stability during extended manipulation periods, helping to maintain consistent experimental conditions. This buffer is membrane-impermeable, has minimal interference with biochemical reactions, and demonstrates strong chemical and enzymatic stability. These properties make it suitable for a wide range of cell culture and biochemical applications. Formulation and Usage The solution is supplied as a 1 M concentrate prepared in cell culture–grade water and is sterile-filtered for use in contamination-sensitive environments. It can be diluted to the desired working concentration depending on application requirements. Store at +2°C to +8°C and handle under aseptic conditions to preserve product integrity. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€52.00

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Non-essential Amino Acids (100x)

Volume: 100 ml Storage: +2°C to +8°C Sterility: Sterile-filtered MEM Non-Essential Amino Acids (100x) is a sterile supplement designed to enhance cell growth and viability in mammalian cell culture systems. The formulation corresponds to a 100x concentrate of the non-essential amino acids found in standard Minimum Essential Medium (MEM), allowing for direct supplementation of basal media with minimal preparation. Application and Benefits This supplement provides an additional amino acid pool for rapidly proliferating cells or for cell lines that have lost the capacity to synthesize non-essential amino acids de novo. By alleviating the metabolic burden of biosynthesis, it supports improved growth kinetics, prolonged viability, and greater experimental consistency—especially in nutrient-sensitive or high-density cultures. Composition and Use The solution includes glycine, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, L-proline, and L-serine. It is compatible with MEM and most other standard media. To use, dilute 1:100 into the final culture medium. This product is sterile-filtered and ready to use without additional handling steps. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€30.00

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PBS

Fosfátový pufrovaný fyziologický roztok (PBS) je široce používaný pufrový roztok v biologickém a chemickém výzkumu. Hraje klíčovou roli při udržování rovnováhy pH a osmolarity během různých experimentálních postupů, včetně zpracování tkání a buněčných kultur. Náš roztok PBS je pečlivě sestaven z vysoce čistých složek, aby byla zajištěna stabilita a spolehlivost při každém experimentu. Osmolarita a koncentrace iontů v našem PBS se velmi blíží hodnotám v lidském těle, díky čemuž je izotonický a pro většinu buněk netoxický. Složení našeho roztoku PBS Náš roztok PBS je směsí fosfátových pufrů a fyziologických roztoků ultračisté kvality s upraveným pH. Při pracovní koncentraci 1X obsahuje: • 8000 mg/l chloridu sodného (NaCl) • 200 mg/l chloridu draselného (KCl) • 1 150 mg/l bezvodého dibasického fosforečnanu sodného (Na₂HPO₄) • 200 mg/l bezvodého monobazického fosforečnanu draselného (KH₂PO₄) Toto složení zajišťuje optimální pH a iontovou rovnováhu, vhodnou pro širokou škálu biologických aplikací. Použití našeho PBS roztoku Náš roztok PBS je ideální pro různé aplikace v biologickém výzkumu. Díky svým izotonickým a netoxickým vlastnostem je vhodný pro ředění látek a proplachování buněčných nádob. Roztoky PBS obsahující EDTA jsou účinné pro uvolnění přilnutých a shluknutých buněk. Do PBS by se však neměly přidávat dvojmocné kovy, jako je zinek, protože to může způsobit srážení. V takových případech se doporučují Goodovy pufry. Navíc je náš roztok PBS přijatelnou alternativou k médiu pro transport virů při přepravě a skladování RNA virů, včetně SARS-CoV-2. Kontrola kvality • Sterilně filtrováno Skladování a trvanlivost • Skladujte při teplotě +2 °C až +25 °C, chráněno před světlem. • Po otevření skladujte při teplotě 2 °C až 25 °C a spotřebujte do 24 měsíců. Přepravní podmínky • Okolní teplota Skladování • Skladujte v chladničce při teplotě +2 °C až +8 °C na tmavém místě. Zabraňte zamrznutí a častému ohřívání na +37 °C, protože to snižuje kvalitu produktu. • Nezahřívejte médium nad 37 °C a nepoužívejte nekontrolované zdroje tepla, jako jsou mikrovlnné trouby. • Pokud má být použita pouze část média, odeberte potřebné množství a před použitím jej ohřejte na pokojovou teplotu. Složení Kategorie Složky Koncentrace (mg/l) Soli Chlorid draselný 200 Bezvodý monobazický fosforečnan draselný 200 Chlorid sodný 8000 Dibasický fosforečnan sodný bezvodý 1150

€20.00

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Sodium Pyruvate Solution (100 mM)

Volume: 100 ml Storage: +2°C to +8°C Sterility: Sterile-filtered Sodium Pyruvate Solution (100 mM) is a sterile, ready-to-use supplement designed to provide an additional, easily accessible energy source for cell culture media. Sodium pyruvate plays a key role in cellular energy metabolism and supports the growth of metabolically active and rapidly proliferating cells, such as tumor cells. Supplementation can enhance cell viability and help maintain metabolic stability in culture systems. Application and Benefits This solution is widely used in routine cell culture to enrich media with pyruvate and promote optimal growth conditions. It supports ATP production, may help reduce oxidative stress, and contributes to improved metabolic performance of cultured cells. Manufactured in cell culture–grade water and sterile-filtered, the product ensures consistent quality and reproducibility in research workflows. Usage and Compatibility The recommended final concentration for most cell culture applications is 1 mM sodium pyruvate, achieved by diluting the 100 mM stock solution 1:100 into complete culture medium. The solution is compatible with a wide range of basal media and mammalian cell lines. Store at +2°C to +8°C and protect from contamination to maintain product stability. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€18.00

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Stable Glutamin (200mM), L-Alanyl-L-Glutamine Solution

Volume: 100 ml Storage: ≤ –15°C Sterility: Sterile-filtered Stable Glutamine Solution (L-Alanyl-L-Glutamine, 200 mM) is a highly stable dipeptide form of L-glutamine designed as a direct replacement for conventional L-glutamine in cell culture media. L-glutamine is an essential amino acid and a major energy source for cultured cells, playing a critical role in cell growth, metabolism, and protein synthesis. Application and Benefits In standard liquid media, L-glutamine degrades relatively quickly at 37°C, leading to the formation of toxic byproducts such as ammonium ions that can negatively affect cell viability and experimental outcomes. Stable glutamine overcomes this limitation by providing a non-degrading dipeptide form that remains intact in culture conditions. Cells enzymatically cleave the dipeptide bond to release L-glutamine as needed, ensuring a continuous fresh supply while preventing the accumulation of harmful waste products. This makes the solution particularly advantageous for long-term cell cultures and high-density growth systems. Formulation and Usage The L-Alanyl-L-Glutamine solution is prepared in cell culture–grade water at a concentration of 200 mM and is sterile-filtered for contamination-sensitive applications. It can be diluted directly into complete media according to experimental requirements. Store at ≤ –15°C and avoid repeated freeze-thaw cycles to maintain product stability. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

€52.00

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