NCI-H647

NCI-H647 cells are a human lung carcinoma cell line derived from a patient with large cell carcinoma of the lung. This cell line is part of the NCI (National Cancer Institute) panel of human tumor cell lines used extensively in cancer research, particularly in studies concerning lung cancer biology and therapeutics.

The NCI-H647 cell line exhibits characteristics typical of large cell lung carcinoma, including rapid growth and the ability to form tumors when xenografted into immunocompromised mice. These cells are particularly useful for exploring the molecular mechanisms of lung cancer pathogenesis, including signal transduction pathways, genetic mutations involved in cancer progression, and the role of tumor microenvironment factors.

NCI-H647 cells are often employed in drug screening studies to evaluate the efficacy and toxicity of chemotherapeutic agents and targeted therapies. Their responsiveness to various anti-cancer compounds helps in understanding the pharmacodynamics and potential resistance mechanisms of lung cancer treatments. This cell line is also used to study the interaction between cancer cells and therapeutic agents, providing insights into the development of more effective and personalized treatment strategies for lung cancer patients.

Overall, the NCI-H647 cell line serves as a critical tool in lung cancer research, facilitating advancements in understanding the disease and developing novel therapeutic approaches.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.