SNU-C1

The SNU-C1 cell line is a human colorectal carcinoma model established from the ascitic fluid of a Korean adult patient. It originates from a moderately differentiated adenocarcinoma of the colon and represents one of a group of SNU-series cell lines derived from colorectal cancer patients. SNU-C1 has been used in numerous studies focused on gastrointestinal cancer biology and pharmacogenomics due to its molecular features and relatively stable growth characteristics under in vitro conditions.

Genomically, SNU-C1 is characterized by microsatellite instability (MSI), a phenotype frequently observed in a subset of colorectal cancers due to defects in the DNA mismatch repair (MMR) system. This MSI status has significant implications for drug sensitivity and genomic instability. Despite harboring multiple genetic alterations common to colorectal carcinoma, including mutations in key pathways such as WNT and p53, SNU-C1 shows distinct proteomic and transcriptomic profiles that make it suitable for molecular subtype classification and high-throughput drug response profiling. It has been included in large-scale datasets such as the Cancer Cell Line Encyclopedia (CCLE), where proteomic quantification confirms expression patterns consistent with epithelial origin and MSI phenotype. These attributes make SNU-C1 a valuable resource for studying therapeutic responses in MSI-high colorectal cancers and for understanding the molecular diversity within colorectal tumors.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.