CBRH7919

The CBRH7919 cell line is a rat hepatoma model frequently utilized in cancer research to investigate liver cancer mechanisms and therapeutic interventions. Derived from hepatocellular carcinoma in rats, these cells are characterized by their rapid proliferation and serve as a useful model for studying hepatocarcinogenesis and liver-specific metabolic functions. Recent studies have highlighted the CBRH7919 cell line's involvement in evaluating the effects of various bioactive compounds on tumor cell growth and signaling pathways.

One significant area of research involving CBRH7919 cells includes the exploration of how natural compounds impact cell proliferation and apoptosis. For instance, choline plasmalogens (ChoPlas) extracted from swine liver have been shown to inhibit the proliferation of CBRH7919 cells. Mechanistically, ChoPlas treatment was found to increase Caveolin-1 expression while reducing phospho-Akt (pAkt) and Bcl-2 levels, thus influencing the PI3K/Akt signaling pathway. This modulation leads to cell cycle arrest at the G1/S transition, accompanied by a decrease in cyclin-dependent kinases (CDK2, CDK4) and cyclins E and D, which are crucial regulators of cell cycle progression.

Additional research has linked the expression of specific proteins like fudenine to metabolic regulation in CBRH7919 cells. Fudenine, a truncated rat homologue of mouse prominin, is reported to be upregulated under high glucose conditions and can increase GAPDH expression in CBRH7919 cells. This suggests that fudenine could play a role in glucose metabolism and potentially influence cellular energy dynamics and stress responses, making CBRH7919 cells a valuable model for diabetes and metabolism-related cancer research.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.