DMS-79

DMS-79 is a human lung cancer cell line derived from a small cell lung carcinoma. These cells exhibit a classical neuroendocrine phenotype, which is characteristic of small cell lung cancer. This phenotype is significant because it implies a potential utility in studying neuroendocrine signaling pathways, which are crucial in the development and progression of lung cancer. The DMS-79 cell line has been widely utilized in research to understand the molecular biology of lung cancers, particularly in the context of tumor genesis, cell proliferation, and apoptosis.

The cell line is known for its aggressive growth and high tumorigenicity in vivo, making it an excellent model for in vivo studies of tumor behavior and response to therapeutics. DMS-79 cells also serve as a useful tool for pharmacological testing and drug development, offering insights into the cellular responses to various chemotherapeutic agents. Furthermore, these cells have been instrumental in the study of cancer stem cell characteristics and mechanisms of metastasis in small cell lung carcinoma. This extensive usage underscores the importance of DMS-79 in cancer research, particularly in therapies targeting aggressive and hard-to-treat cancers like small cell lung carcinoma.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.