KG-1a

The KG-1a cell line is a subline derived from the original KG-1 cell line, which was established from the bone marrow of a patient diagnosed with acute myeloid leukemia (AML). KG-1a cells are classified as a human myeloid leukemia cell line and are particularly characterized by their immature, undifferentiated state. Unlike the parent KG-1 cells, which are primarily in the myeloblast stage, KG-1a cells exhibit a more primitive phenotype, resembling early myeloid progenitors or even stem cells. This makes them an invaluable tool for studying hematopoiesis, leukemia progression, and the molecular mechanisms underlying myeloid differentiation.

KG-1a cells express various surface markers typical of early hematopoietic progenitors, such as CD34, CD38, and HLA-DR, while lacking markers associated with mature myeloid cells. This profile makes them highly suitable for research into stem cell biology and the development of leukemia therapies. Additionally, KG-1a cells are often used in drug screening assays to evaluate the efficacy of potential anti-leukemic compounds, particularly those targeting leukemic stem cells. Their ability to maintain an undifferentiated state in vitro also provides a robust model for gene expression studies and functional assays related to leukemia pathogenesis.

As with other cell lines derived from human tissue, KG-1a cells are intended for research use only and are not suitable for therapeutic or in vivo applications. They require careful handling under sterile conditions, and their growth characteristics necessitate specific culture conditions, including the use of RPMI-1640 medium supplemented with fetal bovine serum. Researchers utilizing the KG-1a cell line can gain significant insights into the early stages of leukemic transformation and the role of hematopoietic progenitors in cancer biology.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.