L-540

L-540 is a human Hodgkin's lymphoma cell line derived from a patient with this form of cancer. This cell line is extensively utilized in research focused on the molecular and cellular mechanisms underlying Hodgkin's lymphoma, a malignancy originating from B lymphocytes. L-540 cells exhibit the characteristic Reed-Sternberg cells, which are a hallmark of Hodgkin's lymphoma and critical for diagnosing this disease. The presence of these multinucleated giant cells makes L-540 an invaluable model for studying the pathophysiology of Hodgkin's lymphoma and for screening potential therapeutic agents targeting these malignant cells.

One of the notable features of L-540 is its expression of CD30, a member of the tumor necrosis factor receptor family, which is often overexpressed in Hodgkin's lymphoma cells. This makes L-540 an excellent model for investigating CD30-targeted therapies, such as antibody-drug conjugates. Additionally, L-540 cells have been used to study the effects of various chemotherapeutic agents and to explore the mechanisms of drug resistance in lymphoma. The cell line’s ability to form tumors in immunocompromised mice further enhances its utility in preclinical studies aimed at evaluating the efficacy of new treatments for Hodgkin's lymphoma.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.