C6-Luc

C6-Luc is a genetically modified derivative of the rat C6 glioma cell line, which was originally established from a N-nitrosomethylurea-induced glial brain tumour in a Wistar rat by Benda et al. (1968). The parental C6 line was stably transfected with a eukaryotic expression plasmid encoding luciferase under control of a constitutive promoter, followed by selection with hygromycin B to obtain stably expressing monoclonal populations.

C6-Luc cells constitutively express luciferase and emit bioluminescent signal in the presence of a luciferin substrate, enabling real-time, non-invasive monitoring of tumour growth by bioluminescence imaging (BLI) in live animals. The line is used to establish orthotopic syngeneic glioma models in Wistar or Sprague-Dawley rats following intracranial stereotactic implantation, and in subcutaneous xenograft models. Applications include evaluation of anti-glioma therapies, drug penetration across the blood-brain barrier, and development of brain tumour imaging platforms.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.