- Tissue
- Ovary
- Disease
- Chinese hamster ovary, non-neoplastic; genetically engineered for BCMA (CD269/TNFRSF17) surface expression
Documents
CHO-BCMA
Cell line · Product number 305972
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01General information
- Description
Disclaimer: The prices displayed for cell lines are exclusively for academic/not-for-profit customers. For commercial entitites the price is approximately €6,250.
If you represent a commercial entity or are unsure which category applies, please contact us.
CHO-BCMA is a stable recombinant Chinese hamster ovary (CHO) cell line engineered to express full-length human B-cell maturation antigen (BCMA; TNFRSF17/CD269). The cell line was generated using a site-specific landing pad recombination system that enables reproducible genomic integration and stable target expression.
BCMA is a transmembrane receptor predominantly expressed on mature B cells and plasma cells, where it regulates cell survival and proliferation through interaction with the ligands BAFF and APRIL. The receptor is highly relevant in multiple myeloma and other B-cell malignancies, making CHO-BCMA cells useful for therapeutic antibody screening, CAR-T and bispecific T-cell engager development, receptor binding studies, and flow cytometry-based assay development.
- Tissue
- Ovary
- Disease
- Chinese hamster ovary, non-neoplastic; genetically engineered for BCMA (CD269/TNFRSF17) surface expression
- Applications
- Antibody screening; BCMA-targeted therapy development; CAR-T cell validation; multiple myeloma research; flow cytometry
02Classification
- Organism
- Chinese hamster
- Growth properties
- Adherent and suspension
- GMO status
- GMO-S1
- GMO status details
- GMO-S1: This CHO cell line contains a BCMA expression cassette supporting receptor-function analyses. This classification applies only within Germany and may differ elsewhere.
- Biosafety level
- 1
03Characteristics
- Morphology
- Epithelial-like
- Cell type
- Epithelial cells
- Doubling time
- approx. 14-16 hours
04Identifiers
- Citation
- CHO-BCMA (Cytion catalog number 305972)
- NCBI TaxID
- 10029
- Cellosaurus
- CVCL_A8V3
05Expression and susceptibility
- Receptors expressed
- BCMA, TNFRSF17/CD269
06Handling and culture
- Culture medium
For adherent cultures: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039)
- Incubation atmosphere
37°C, 5% CO2, humidified atmosphere.
- Supplements
- For adherent cultures: Supplement the medium with 5% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL.
- Dissociation reagent
- For adherent cultures: Trypsin-EDTA
- Subculturing
- For routine adherent cell culture: Aspirate the old culture medium from the adherent cells, and wash them with PBS to remove any remaining medium. After aspirating the PBS, add the appropriate volume of Trypsin/EDTA solution based on the culture vessel size (e.g., 1 ml for a T25 flask, 3 ml for a T75 flask) and incubate at room temperature or 37°C for 5-10 minutes, or until the cells detach. Monitor detachment under a microscope, and gently tap the vessel if necessary to release the cells. Once detached, add complete medium to inactivate the Trypsin/EDTA, gently resuspend the cells, and transfer an aliquot of the cell suspension into a new culture vessel containing fresh medium. Place the vessel in an incubator set to 37°C with 5% CO2, and change the medium every 2-3 days.
- Split ratio
- A ratio of 1:5 to 1:10 is recommended
- Seeding density
- 2 to 4 x 105 cells/mL
- Fluid renewal
- 2 to 3 times per week
07Legal and safety
- Material Transfer Agreement required
- Yes