BV-173 细胞

BV-173 细胞系源自 1980 年确诊为费城染色体阳性（Ph+）慢性髓性白血病（CML）患者的外周血。这种细胞系因其 Ph+ 状态而备受关注，Ph+ 状态表明一种特殊的染色体异常，涉及 9 号染色体和 22 号染色体之间的易位。这种易位通常被称为费城染色体，会导致 BCR-ABL 融合基因，这是一种关键的分子标志，通过促进白血病细胞的增殖和存活来驱动 CML 的发病机制。

BV-173 细胞在血液学研究中被广泛用作研究 CML 细胞和分子机制的模型，特别是在耐药性和细胞对酪氨酸激酶抑制剂（TKIs）的反应方面，TKIs 是 BCR-ABL 融合蛋白的靶向药物。该细胞系在临床前研究中发挥了重要作用，有助于评估新的治疗策略和了解 CML 的生物学特性。BV-173 具有髓系细胞的典型特征，通常用于研究 CML 中因 BCR-ABL 致癌基因而失调的信号转导通路。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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