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Hey
Tissue
卵巢
Disease
高级别卵巢浆液性腺癌
Synonyms
HEY

Documents

Hey

Cell line · Product number 305017

HEY Cells, derived from a human ovarian cancer xenograft, are a valuable resource for cancer researchers seeking to advance their understanding of papilla...

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01General information

Description

HEY 细胞来源于人类卵巢癌异种移植,是癌症研究人员了解乳头状囊腺癌(一种中度分化的卵巢癌)的宝贵资源。亲本细胞系 HEY 最初是从一名确诊患有这种特殊类型癌症的高加索患者的腹膜样本中获得的。这些上皮样细胞与人体细胞非常相似,因此是研究卵巢癌的绝佳模型。HEY,细胞的快速倍增时间约为 30 小时,可进行高效、省时的实验。研究人员可以利用这些细胞研究癌症生物学的各个方面,如肿瘤形成、转移和药物反应。

HEY细胞特别适合涉及三维细胞培养的应用,这种技术更接近肿瘤的生理环境。它们能在半固体培养物中生长,并能作为异种移植物在免疫剥夺的 CBA/CJ 小鼠体内生长,这突出表明了它们在体内研究方面的适应性和潜力。通过将 HEY 细胞纳入癌症研究,科学家们可以发现乳头状膀胱腺癌发展和恶化的重要线索。这些细胞对于探索新的治疗策略、确定潜在的药物靶点和评估治疗效果非常宝贵。

总之,HEY 细胞为研究人员提供了强大而可靠的卵巢癌研究资源。这些细胞来源于患者样本,具有上皮样形态,能忠实复制乳头状膀胱腺癌的关键特征。它们在三维细胞培养和癌症研究中的应用,使它们成为推动我们了解这种具有挑战性的疾病的关键。

Tissue
卵巢
Disease
高级别卵巢浆液性腺癌
Synonyms
  • HEY

02Classification

Organism
Human
Sex
Female
Growth properties
Adherent
Biosafety level
1

03Characteristics

Morphology
上皮细胞
Tumourigenic
是
Doubling time
20 至 30 小时

04Identifiers

Citation
嘿(Cytion 产品编号 305017)
NCBI TaxID
9606
Cellosaurus
CVCL_0297

05Handling and culture

Culture medium
DMEM,w:4.5 克/升葡萄糖,w:4 毫摩尔 L-谷氨酰胺,w:3.7 克/升 NaHCO3,w:1.0 毫摩尔丙酮酸钠(Cytion 编号 820300a)。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% FBS
Dissociation reagent
Accutase
Subculturing
去除粘附细胞上的旧培养基,用不含钙和镁的 PBS 冲洗。T25 烧瓶用 3-5 毫升 PBS,T75 烧瓶用 5-10 毫升。然后用 Accutase 完全覆盖细胞,T25 烧瓶用 1-2 毫升,T75 烧瓶用 2.5 毫升。让细胞在室温下孵育 8-10 分钟,使其分离。孵育后,用 10 毫升培养基轻轻混合细胞使其重悬,然后用 300xg 离心 3 分钟。弃去上清液,用新鲜培养基重悬细胞,并将其转移到已装有新鲜培养基的新烧瓶中。
Thawing and culturing
  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

  7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

  8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

06Quality control and cryopreservation

Sterility

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染,每天都要对细胞培养物进行目视检查。

Freeze medium
我们使用完全生长培养基(包括 FBS)+10% DMSO 或 CM-1(Cytion 商品目录编号 800100)作为冷冻保存培养基,以获得足够的解冻后存活率,CM-1(Cytion 商品目录编号 800100)含有优化的渗透保护剂和代谢稳定剂,可提高恢复能力并减少冷冻引起的应激。
Storage conditions

如需长期保存,可将样品瓶放入气相液氮中,温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运,并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后,应立即检查容器,并立即将小瓶转移到适当的储藏室。

07Donor

Donor age
未说明
Ethnicity
欧洲