KLN-205

KLN-205 是一种小鼠肺癌细胞系，来源于成年小鼠。这种细胞系被广泛用于癌症研究，特别是用于研究肺癌进展、转移和潜在治疗干预的机制。KLN-205 细胞具有非小细胞肺癌 (NSCLC) 的典型特征，是研究这种疾病的分子和细胞基础的宝贵模型。研究人员利用 KLN-205 来评估各种化疗药物、免疫疗法和靶向治疗的疗效，帮助加深对肺癌生物学和治疗策略的了解。

KLN-205 细胞以其生长旺盛和植入免疫受损小鼠体内后能形成肿瘤而著称，为临床前研究提供了可靠的体内模型。这些细胞用于探索肿瘤与宿主的相互作用、肺癌的免疫反应以及基因和表观遗传修饰对癌症发展和恶化的影响。KLN-205 细胞系是肿瘤学研究的重要工具，有助于确定肺癌的新型生物标记物和治疗靶点。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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